The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture
The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture
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DOI：
10.1016/j.biomaterials.2005.01.006
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发表时间：
2005-08-01
期刊：
影响因子：
14
通讯作者：
Tanaka, J
中科院分区：
文献类型：
--
作者：
Maeno, S;Niki, Y;Tanaka, J
我们的研究小组旨在开发一种骨软骨复合材料，使用II型胶原凝胶与羟基磷灰石（HAp）沉积在一侧。浸泡在Ca 2+和磷酸盐溶液中的凝胶是必不可少的HAp沉积，所以细胞行为和Ca 2+浓度之间的关系进行了研究，在二维和三维的文化。目前的结果表明，2-4 mM的Ca 2+是适合于成骨细胞的增殖和生存，而略高浓度（6-8 mM）有利于成骨细胞分化和基质矿化的2-和3-维文化。较高浓度（> 10 mM）具有细胞毒性。从钙沉积的角度来看，较高浓度的磷酸盐导致Cat（2+）的积累增加。在含磷酸盐的凝胶中培养细胞在含Ca 2+的培养基中也导致凝胶中HAp的时间依赖性形成。考虑到包埋细胞的活力，在Ca 2+浓度约为5 mM的培养基中培养支架对于HAp沉积和成骨细胞行为都是有用的。(C)2005爱思唯尔有限公司保留所有权利。
Our research group aims to develop an osteochondral composite using type II collagen gel with hydroxyapatite (HAp) deposited on one side. Soaking gels in Ca2+ and phosphate solution is indispensable to HAp deposition, so relationships between cell behavior and Ca2+ concentration were examined in two- and three-dimensional cultures. The present results indicate that 2-4 mM Ca2+ is suitable for proliferation and survival of osteoblasts, whereas slightly higher concentrations (6-8 mM) favor osteoblast differentiation and matrix mineralization in both 2- and 3-dimensional cultures. Higher concentrations (> 10 mM) are cytotoxic. Purely from the perspective of calcium deposition, higher concentrations lead to increased accumulation of Cat(2+).Culturing cells in phosphate-containing gel in media with Ca2+ also leads to time-dependent formation of HAp in the gel. Considering the viability of embedded cells, culturing scaffolds in media with Ca2+ concentrations around 5 mM is useful for both HAp deposition and osteoblast behavior. (C) 2005 Elsevier Ltd. All rights reserved.