The inhibition of progesterone secretion and the regulation of cyclic nucleotides by atrial natriuretic factor in gonadotropin responsive murine Leydig tumor cells.
The inhibition of progesterone secretion and the regulation of cyclic nucleotides by atrial natriuretic factor in gonadotropin responsive murine Leydig tumor cells.
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促性腺激素反应性鼠 Leydig 肿瘤细胞中心房钠尿因子对孕酮分泌的抑制和环核苷酸的调节。
DOI：
10.1016/0006-291x(85)90975-1
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发表时间：
1985
影响因子：
3.1
通讯作者：
Inagami,T
中科院分区：
文献类型：
--
作者：
Pandey,KN;Kovacs,WJ;Inagami,T
我们发现心房利钠因子（ANF）在改变细胞内环核苷酸水平以及孕酮分泌方面对睾丸细胞有深远的影响。使用克隆培养的Leydig肿瘤细胞，我们发现与对照组相比，1×10− 8 M ANF导致细胞cGMP积累增加2000倍，并抑制处理细胞中cAMP超过90%。ANF（1×10− 8 M）也显著抑制促性腺激素刺激的cAMP对牛促黄体激素（bLH）或人绒毛膜促性腺激素（hCG）的反应。在这些培养的Leydig肿瘤细胞中，促性腺激素刺激的孕酮分泌被ANF（1×10−10-1×10− 9 M）抑制。在1×10− 9 M的肽浓度下观察到孕酮分泌约50%的抑制。
We have found that atrial natriuretic factor (ANF) has a profound effect on testicular cells in altering intracellular cyclic nucleotide levels as well as progesterone secretion. Using clonal cultured Leydig tumor cells we found that 1×10−8M ANF caused a two thousand-fold elevation in the accumulation of cellular cGMP and inhibited cAMP in treated cells by more than 90% as compared to the controls. ANF (1×10−8M) also significantly inhibited gonadotropin-stimulated accumulation of cAMP in response to bovine luteinizing hormone (bLH) or human chorionic gonadotropin (hCG). Gonadotropin-stimulated progesterone secretion was inhibited by ANF (1×10−10–1×10−9M) in these cultured Leydig tumor cells. Approximately 50% inhibition of progesterone secretion was observed at the peptide concentration of 1×10−9M.