Functional expression of CXCR3 in cultured mouse and human astrocytes and microglia
Functional expression of CXCR3 in cultured mouse and human astrocytes and microglia
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DOI：
10.1016/s0306-4522(02)00114-8
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发表时间：
2002-01-01
期刊：
影响因子：
3.3
通讯作者：
Boddeke, HWGM
中科院分区：
文献类型：
--
作者：
Biber, K;Dijkstra, I;Boddeke, HWGM
最近已经确定，中枢神经系统的炎症伴随着中枢神经系统内趋化因子的表达。多项证据表明，中枢神经系统内的趋化因子启动并协调血液白细胞对发炎大脑的浸润。还已知中枢神经系统的内源性细胞表达功能性趋化因子受体，这增加了趋化因子可能参与脑炎症期间脑细胞之间的细胞间信号传导的可能性。最近的研究表明，CXCR3 的两种趋化因子配体在受损神经元中被快速诱导。目前对神经元趋化因子表达的功能知之甚少。为了研究神经元趋化因子是否有助于中枢神经系统内的内源性信号传导，我们研究了 CXCR3 在神经胶质细胞中的可能表达。逆转录聚合酶链反应实验和原位杂交分析表明，培养的小鼠和人类来源的星形胶质细胞和小胶质细胞均表达 CXCR3 mRNA。通过免疫细胞化学检测两种细胞类型中CXCR3的蛋白表达。此外，用CXCR3的趋化因子配体刺激培养的神经胶质细胞诱导细胞内钙瞬变和趋化性，表明CXCR3的功能性表达。这些结果表明培养物中的神经胶质细胞功能性地表达趋化因子受体CXCR3。由于已经表明脑损伤会迅速诱导激活 CXCR3 的神经元趋化因子的表达，因此我们认为神经胶质 CXCR3 可能有助于中枢神经系统中与病理状况相关的细胞间信号系统。 (C) 2002 国际广播组织。由爱思唯尔科学有限公司出版。保留所有权利。
It has been established recently that inflammation of the CNS is accompanied by an expression of chemokines within the CNS. Several lines of evidence suggest that chemokines within the CNS initiate and orchestrate the infiltration of the inflamed brain by blood leukocytes. It is also known that endogenous cells of the CNS express functional chemokine receptors, raising the possibility that chemokines may be involved in intercellular signalling between brain cells during brain inflammation. It was shown recently that two chemokine ligands for CXCR3 are induced rapidly in damaged neurons. Little is known yet on the function of neuronal chemokine expression. In order to investigate whether neuronal chemokines contribute to endogenous signalling within the CNS we investigated possible expression of CXCR3 in glial cells. Reverse transcription-polymerase chain reaction experiments and in situ hybridization analysis showed that cultured astrocytes and microglia from both mouse and human sources express CXCR3 mRNA. Protein expression of CXCR3 in both cell types was detected by immunocytochemistry. Moreover, stimulation of cultured glial cells with chemokine ligands for CXCR3 induced intracellular calcium transients and chemotaxis, indicating the functional expression of CXCR3.These results indicate that glial cells in culture functionally express the chemokine receptor CXCR3. Since it has been shown that brain damage rapidly induces expression of neuronal chemokines that activate CXCR3, we suggest that glial CXCR3 might contribute to an intercellular signalling system in the CNS related to pathological conditions. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.