Sample preparation: the weak link in microfluidics-based biodetection
Sample preparation: the weak link in microfluidics-based biodetection
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DOI：
10.1007/s10544-008-9190-7
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发表时间：
2008-12-01
影响因子：
2.8
通讯作者：
Mariella, Raymond, Jr.
中科院分区：
文献类型：
--
作者：
Mariella, Raymond, Jr.
作为一个广泛的概括，临床医生和实验室人员谁使用基于微流体的自动化或半自动化仪器来执行生物医学分析对现实世界的样品更满意的状态，比他们与前端样品制备的状态。端到端的过程需要一个人来收集，操作，制备和分析样品。微流体技术对该过程的吸引力部分基于其小尺寸和处理非常小的液体体积的能力的组合，从而最大限度地减少了可能昂贵的试剂的使用。然而，真实世界的样品通常是大的，并且与微流体装置的输入端口和μ m级通道不兼容，并且非常小的液体体积在分析低浓度的目标分析物时可能是不合适的。获取原始样品，将其引入基于微流体的系统中，并进行样品制备，这可能包括目标分析物的分离和浓缩，这可能是一个有价值的挑战，以便人们可以从试剂保存的小体积中受益，并在最终实施感兴趣的测定时获得正确的答案。
As a broad generalization, clinicians and laboratory personnel who use microfluidics-based automated or semi-automated instrumentation to perform biomedical assays on real-world samples are more pleased with the state of the assays than they are with the state of the front-end sample preparation. The end-to-end procedure requires one to collect, manipulate, prepare, and analyze the sample. The appeal of microfluidics for this procedure is partly based on its combination of small size and its ability to process very small liquid volumes, thus minimizing the use of possibly expensive reagents. However, real-world samples are often large and incompatible with the input port and the mu m-scale channels of a microfluidic device, and very small liquid volumes can be inappropriate in analyzing low concentrations of target analytes. It can be a worthy challenge to take a raw sample, introduce it into a microfluidics-based system, and perform the sample preparation, which may include separation and concentration of the target analytes, so that one can benefit from the reagent-conserving small volumes and obtain the correct answer when finally implementing the assay of interest.