Chemical profiling and gene expression profiling during the manufacturing process of Taiwan oolong tea "oriental beauty"
Chemical profiling and gene expression profiling during the manufacturing process of Taiwan oolong tea "oriental beauty"
复制标题
DOI：
10.1271/bbb.60708
复制
发表时间：
2007-06-01
影响因子：
1.6
通讯作者：
Sakata, Kanzo
中科院分区：
文献类型：
--
作者：
Cho, Jeong-Yong;Mizutani, Masaharu;Sakata, Kanzo
东方美人茶，是由台湾产的绿色茶蝉（Jacobiasca formosana）寄生的茶叶制成，具有成熟水果和蜂蜜的独特香气。为了确定在乌龙茶制造过程中茶叶中发生了什么，研究了基因表达谱和化学谱。茶叶样品是从茶树品种。中华树当茶叶被昆虫攻击时，金新大胖。主要挥发性化合物，如芳樟醇氧化物、苯甲醇、2-苯基乙醇和2，6-二甲基辛-3，7-二烯-2，6-二醇，在生产过程中增加。通过对新鲜叶和第一次翻转的茶叶进行差异筛选，分析了加工过程中的基因表达谱。许多上调的转录本被发现编码与应激反应蛋白同源的各种蛋白质。相应地，内源脱落酸和棉子糖的含量在生产过程中增加。因此，传统的制造方法是一种独特的过程，利用植物防御反应来提高挥发性化合物和其他代谢产物的产量。
Oriental Beauty, which is made from tea leaves infested by the tea green leafhopper (Jacobiasca formosana) in Taiwan, has a unique aroma like ripe fruits and honey. To determine what occurs in the tea leaves during the oolong tea manufacturing process, the gene expression profiles and the chemical profiles were investigated. Tea samples were prepared from Camellia sinensis var. sinensis cv. Chin-shin Dah-pang while the tea leaves were attacked by the insect. The main volatile compounds, such as linalool-oxides, benzyl alcohol, 2-phenylethanol, and 2,6-dimethylocta-3,7-diene-2,6-diol, increased during manufacture. The gene expression profiles during manufacture were analyzed by differential screening between fresh leaves and tea leaves of the first turn over. Many up-regulated transcripts were found to encode various proteins homologous to stress response proteins. Accordingly, the endogenous contents of abscisic acid and raffinose increased during manufacture. Thus the traditional manufacturing method is a unique process that utilizes plant defense responses to elevate the production of volatile compounds and other metabolites.