SEQUENCE OF EVENTS IN THE DIGESTION OF FRESH LEGUME LEAVES BY RUMEN BACTERIA
SEQUENCE OF EVENTS IN THE DIGESTION OF FRESH LEGUME LEAVES BY RUMEN BACTERIA
复制标题
DOI：
10.1128/aem.40.3.613-625.1980
复制
发表时间：
1980-01-01
影响因子：
4.4
通讯作者：
COSTERTON, JW
中科院分区：
文献类型：
--
作者：
CHENG, KJ;FAY, JP;COSTERTON, JW
当新鲜的全叶6种不同的饲料豆类悬浮在人工[牛]瘤胃培养基和接种瘤胃细菌，细菌粘附和增殖，注意到在气孔，这些细菌的气孔渗透记录EM。入侵的细菌粘附在叶片的细胞间隙内的表面上，并产生非常广泛的胞外多糖封闭的小菌落。在一些豆科植物叶细胞壁被细菌消化破坏和破裂后，这些细胞（特别是薄壁细胞和表皮细胞）被细菌侵入，随后形成细胞内小菌落。其他细胞既不破裂也不定植（特别是气孔保卫细胞和维管组织）。在消化完整的豆科植物叶片的所有阶段，瘤胃细菌生长在由单一或混合形态类型的细胞组成的小菌落中;特定的生态位通常完全且一致地被单一混合形态类型的细胞的非常大的小菌落占据。
When fresh whole leaves of 6 different species of forage legumes were suspended in an artificial [cow] rumen medium and inoculated with rumen bacteria, bacterial adhesion and proliferation were noted at the stomata, and penetration of the stomata by these bacteria was documented by EM. The invading bacteria adhered to surfaces within the intercellular space of the leaf and produced very extensive exopolysaccharide-enclosed microcolonies. After some of the legume leaf cell walls were disorganized and ruptured by bacterial digestion, these cells (notably, parenchyma and epidermal cells) were invaded by bacteria, with subsequent formation of intracellular microcolonies. Other cells were neither ruptured nor colonized (notably, stomata guard cells and vascular tissue). At all stages of the digestion of intact legume leaves, the rumen bacteria grew in microcolonies composed of cells of single or mixed morphological types; a particular ecological niche was often completely and consistently occupied by a very large microcolony of cells of single mixed morphological types.