Genetic diversity and biogeography of rhizobia associated with Caragana species in three ecological regions of China
Genetic diversity and biogeography of rhizobia associated with Caragana species in three ecological regions of China
复制标题
中国三个生态区柠条属根瘤菌遗传多样性及生物地理学
DOI：
10.1016/j.syapm.2008.10.004
复制
发表时间：
2009-08-01
影响因子：
3.4
通讯作者：
Chen, Wen Xin
中科院分区：
文献类型：
--
作者：
Lu, Yang Li;Chen, Wen Feng;Chen, Wen Xin
从锦鸡儿属植物中分离到的174株根瘤菌中，有22个基因种主要属于中慢生根瘤菌属（Mesorhizobium），偶尔也有根瘤菌属（Rhizobium）和慢生根瘤菌属（Bradyrhizobium）。在慢生根瘤菌和所有检测到的中慢生根瘤菌菌株中发现了高度相似的cDNAC基因。同源性分析表明，根瘤菌基因种与其所处的生态区域之间存在明显的相关性。这些结果表明，锦鸡儿属物种对根瘤菌共生基因型有严格的选择性，但对基因组背景没有明显的选择性;锦鸡儿属根瘤菌存在共生基因从中慢生根瘤菌到中慢生根瘤菌以及种间的横向转移;锦鸡儿属根瘤菌存在植物地理学。此外，根据16 S rRNA基因扩增、16 S-23 S基因间区限制性内切酶分析、BOX PCR和蛋白质SDS-PAGE分析的结果进行聚类分析，是鉴定基因种的有效方法。(C)2008年Elsevier GmbH。All rights reserved.
Twenty-two genospecies belonging mainly to Mesorhizobium, and occasionally to Rhizobium and Bradyrhizobium, were defined among the 174 rhizobia strains isolated from Caragana species. Highly similar nodC genes were found in the sole Bradyrhizobium strain and among all the detected Mesorhizobium strains. A clear correlation between rhizobial genospecies and the eco-regions where they were isolated was Found using homogeneity analysis. All these results demonstrated that Caragana species had stringently selected the rhizobia symbiotic genotype, but not the genomic background; lateral transfer of symbiotic genes from Mesorhizobium to Bradyrhizobium and among the Mesorhizobium species has happened in the Caragana rhizobia; and biogeography of Caragana rhizobia exists. Furthermore, a combined cluster analysis, based upon the patterns obtained from amplified 16S rRNA gene and 16S-23S intergenic spacer restriction analyses, BOX PCR and SDS-PAGE of proteins, was reported to be an efficient method to define the genospecies. (C) 2008 Elsevier GmbH. All rights reserved.