Spatial segregation within the sacral parasympathetic nucleus of neurons innervating the bladder or the penis of the rat as revealed by three-dimensional reconstruction
Spatial segregation within the sacral parasympathetic nucleus of neurons innervating the bladder or the penis of the rat as revealed by three-dimensional reconstruction
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DOI：
10.1016/s0306-4522(02)00405-0
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发表时间：
2002-01-01
期刊：
影响因子：
3.3
通讯作者：
Maurin, Y
中科院分区：
文献类型：
--
作者：
Banrezes, B;Andrey, P;Maurin, Y
本研究的目的是：（1）检查成年雄性大鼠腰骶脊髓骶副交感神经核节前神经元的空间结构;（2）在该核中，寻找分别支配阴茎或膀胱的神经元亚群的可能分离。为了估计它们的空间组织，用麦胚凝集素偶联辣根过氧化物酶逆行标记骶骨副交感神经核的神经元，该酶应用于切片的骨盆神经的中央端。本研究采用注射伪狂犬病病毒的跨突触逆行标记法，对支配阴茎海绵体和膀胱穹隆的腰骶神经元进行了亚群鉴定。在麦胚凝集素标记和伪狂犬病病毒标记的大鼠中，通过脊髓L5-S1节段切割连续冠状切片。通过组织化学（过氧化物酶实验）或免疫组织化学（伪狂犬病毒实验）显示标记的神经元。通过我们实验室开发的三维重建软件，从每个脊柱切片图像系列计算三维模型。他们揭示了（i）节前神经元和（ii）支配膀胱或阴茎的神经元的空间组织。随后将不同的三维模型合并为一个单一的模型，该模型揭示了骶副交感神经核内神经元亚群的分离。注射入阴茎的病毒标记神经元主要从L 6节的吻侧部延伸到S1节的吻侧部，而注射入膀胱的标记神经元主要从L 6节的尾侧部延伸到S1节的尾侧部，这些结果支持骶神经元的内脏定位组织提供骨盆器官的脊髓控制的假说。(C)2002年IBRO。由Elsevier Science Ltd.出版，版权所有。
The purpose of the present investigations was (1) to examine the spatial organization of preganglionic neurons of the sacral parasympathetic nucleus in the lumbosacral spinal cord of male adult rats and (2) to search, in this nucleus, for a possible segregation of sub-populations of neurons innervating the penis or the bladder, respectively. To estimate their spatial organization, neurons of the sacral parasympathetic nucleus were retrogradely labeled by wheat germ agglutinin coupled to horseradish peroxidase applied to the central end of the sectioned pelvic nerve. The sub-populations of lumbosacral neurons innervating the corpus cavernosum of the penis or the dome of the bladder were identified using transsynaptic retrograde labeling by pseudorabies virus injected into these organs in different rats. In both wheat germ agglutinin-labeled and pseudorabies virus-labeled rats, serial coronal sections were cut through the spinal L5-S1 segments. Labeled neurons were revealed by histochemistry (peroxidase experiments) or immunohistochemistry (pseudorabies virus experiments). By means of a three-dimensional reconstruction software developed in our laboratory, three-dimensional models were calculated from each spinal section image series. They revealed the spatial organization of (i) preganglionic neurons and (ii) neurons innervating the bladder or the penis. The different three-dimensional models were subsequently merged into a single one which revealed the segregation, within the sacral parasympathetic nucleus, of the sub-populations of neurons. Neurons labeled by virus injected into the penis extended predominantly from the rostral part of the L6 segment to the rostral part of the S1 segment while those labeled by bladder injections were distributed predominantly from the caudal part of the L6 segment to the caudal part of the S1 segment.These results support the hypothesis of a viscerotopic organization of sacral neurons providing the spinal control of pelvic organs. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.