Suppression of inducible nitric oxide production by indole and isothiocyanate derivatives from Brassica plants in stimulated macrophages
Suppression of inducible nitric oxide production by indole and isothiocyanate derivatives from Brassica plants in stimulated macrophages
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DOI：
10.1055/s-2003-42790
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发表时间：
2003-08-01
期刊：
影响因子：
2.7
通讯作者：
Chang, HP
中科院分区：
文献类型：
--
作者：
Chen, YH;Dai, HJ;Chang, HP
本研究探讨了芸苔属蔬菜中的活性化合物2-苯乙基异硫氰酸酯（PEITC）、吲哚-3-甲醇（13 C）和吲哚并[3，2-B]咔唑（ICZ）对RAW 264.7细胞NO生成的抑制作用。结果表明，PEITC和13 C抑制脂多糖（LPS）和干扰素-γ（IFN-γ）诱导的RAW 264.7细胞NO的产生，这种抑制作用与降低iNOS蛋白和mRNA的表达一致。相反，ICZ，13 C的衍生物，刺激NO的产生没有显着的影响。总之，芸苔属植物衍生物，PEITC和，在较小程度上，13 C通过降低RAW 264.7细胞中iNOS蛋白和mRNA的表达来抑制LPS/IFN-γ诱导的NO产生，其中PEITC具有更有效的抑制作用。然而，ICZ对活化的NO产生没有抑制作用（吲哚-3-甲醇=吲哚-3-甲醇）。
In this study, the effects of bioactive compounds derived from vegetables of the Brassica genus (Brassicaceae) including 2-phenylethyl isothiocyanate (PEITC), indole-3-carbinol (13C), and indolo[3,2-b]carbazole (ICZ), on the inhibition of NO production in RAW 264.7 cells were explored. The results indicated that PEITC and 13C inhibited lipopolysaccharide (LPS)- and interferon-gamma (IFN-gamma)-induced NO production in RAW 264.7 cells, and this inhibition was in accordance with lowering the expression of iNOS protein and mRNA. On the contrary, ICZ, a derivative of 13C, had no significant effect on the stimulated NO production. In conclusion, the Brassica plants derivatives, PEITC and, to a lesser extent, 13C inhibit the LPS/IFN-gamma-induced NO production by lowering iNOS protein and mRNA expression in RAW 264.7 cells, in which the PEITC had a more potent inhibitory effect. Nevertheless, ICZ exhibits no inhibitory effect on the activated NO production (Indole-3-carbinol = indole-3-methanol).