Use of antibodies to RCC and CD10 in the differential diagnosis of renal neoplasms
Use of antibodies to RCC and CD10 in the differential diagnosis of renal neoplasms
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DOI：
10.1097/00000478-200002000-00006
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发表时间：
2000-02-01
影响因子：
5.6
通讯作者：
Corless, CL
中科院分区：
文献类型：
--
作者：
Avery, AK;Beckstead, J;Corless, CL
大多数肾肿瘤仅根据组织学检查即可区分。然而，透明细胞肾癌和嫌色细胞癌之间以及这些肿瘤的颗粒/嗜酸性变体和肾嗜酸细胞瘤之间存在形态学相似性。只有有限数量的组织化学标记物可用于帮助这些肿瘤的鉴别诊断。黑尔胶体铁通常对嫌色细胞癌产生强烈的、弥漫性的细胞质染色，而透明细胞癌通常呈阴性。然而，对这种污点的解释并不总是那么简单。通过免疫组织化学，波形蛋白在大多数透明细胞癌中可检测到，而在大多数嫌色细胞肿瘤和嗜酸细胞瘤中不存在，但依赖单一抗体可能会产生误导。在本报告中，我们研究了市售的 RCC 和 CD10 单克隆抗体在常见肾肿瘤鉴别诊断中的应用。 85% 的透明细胞癌（62 例中的 53 例）具有可检测到的 RCC 表面膜染色，94%（62 例中的 58 例）呈 CD10 阳性。几乎所有病例中乳头状癌的 RCC 和 CD10 均呈强阳性（14 例中各 13 例）。相比之下，所有 19 个嫌色细胞癌的表面膜染色均呈完全阴性。嗜酸细胞瘤的 RCC 也呈阴性（9 例中的 0 例），但在某些病例中可检测到 CD10（9 例中的 3 例）。这些结果表明，RCC 和 CD10 表面膜染色的存在可用于确认疑似透明细胞癌或乳头状肾癌的诊断。嫌色细胞癌的两种标记物均应呈阴性。 RCC 染色缺失也可能有助于诊断肾嗜酸细胞瘤。
The majority of renal neoplasms can be distinguished on the basis of histologic examination alone; however, there are morphologic similarities between clear cell renal carcinoma and chromophobe cell carcinoma, as well as between the granular/ eosinophilic variants of these tumors and renal oncocytoma. Only a limited number of histochemical markers are available to aid in the-differential diagnosis of these neoplasms. Hale's colloidal iron usually yields strong, diffuse cytoplasmic staining of chromophobe cell carcinomas whereas clear cell carcinomas are generally negative; however, interpretation of this stain is not always straightforward. By immunohistochemistry, vimentin is detectable in most clear cell carcinomas and is absent from most chromophobe cell tumors and oncocytomas, but reliance on a single antibody can be misleading. In this report we examine the use of commercially available monoclonal antibodies to RCC and CD10 in the differential diagnosis of common renal tumors. Eighty-five percent of clear cell carcinomas (53 of 62) had detectable surface membrane staining for RCC, and 94% (58 of 62) were positive for CD10. Papillary carcinomas were likewise strongly positive for RCC and CD10 in nearly all cases (13 of 14 each). In contrast, all 19 chromophobe cell carcinomas examined were completely negative for surface membrane staining with both of these markers. Oncocytomas were also negative for RCC (0 of 9), but CD10 was detectable in some cases (3 of 9). These results suggest that the presence of surface membrane staining for RCC and CD10 may be used to confirm a diagnosis of suspected clear cell or papillary renal carcinoma. Chromophobe cell carcinomas should be negative for both markers. The absence of RCC staining may also be helpful in the diagnosis of renal oncocytoma.