Biocompatibility of poly(D,L-lactide-co-glycolide) nanoparticles conjugated with alendronate
Biocompatibility of poly(D,L-lactide-co-glycolide) nanoparticles conjugated with alendronate
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DOI：
10.1016/j.biomaterials.2007.12.022
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发表时间：
2008-04-01
期刊：
影响因子：
14
通讯作者：
Baldini, Nicola
中科院分区：
文献类型：
--
作者：
Cenni, Elisabetta;Granchi, Donatella;Baldini, Nicola
采用乳化/溶剂挥发法制备了丙交酯-乙交酯共聚物-阿仑膦酸钠复合物纳米粒。通过MALDI TOF分析测定的缀合产率为30- 35%。通过光子相关光谱法评估的PLGA-ALE NP尺寸为198.7 +/-0.2nm。与用磷酸盐缓冲盐水（PBS）孵育的血液相比，使用不同浓度PLGA-ALE NP的血液相容性研究未显示对溶血、白细胞数量、血小板活化、APTT和补体消耗的任何显著影响。在与560 μ g/ml PLGA-ALE NP孵育后，证明凝血酶原活性显著降低;在最高稀释度下观察到显著增加。通过中性红试验评价的人脐静脉内皮细胞和骨髓基质细胞（BMSC）的活力不受PLGA-ALE NP的影响。在加入PLGA-ALE NP和PBS的培养基中培养的BMSC之间，细胞相关碱性磷酸酶无显著差异。这些结果表明PLGA-ALE NP具有可接受的血液相容性程度，并且没有细胞毒性;因此，它们可能被认为适合静脉给药。(c)2007爱思唯尔有限公司保留所有权利。
Nanoparticles made of a conjugate Of Poly(D,L-lactide-co-glycolide) with alendronate (PLGA-ALE NPs), were prepared by emulsion/solvent evaporation technique. The conjugation yield, determined by MALDI TOF analysis, was 30-35%. PLGA-ALE NPs size, evaluated by photon correlation spectroscopy, was 198.7 +/- 0.2 nm. Haemocompatibility studies using different concentrations of PLGA-ALE NPs did not show any significant effect on haemolysis, leukocyte number, platelet activation, APTT and complement consumption, in comparison with blood incubated with phosphate buffered saline (PBS). A significant reduction of the prothrombin activity was demonstrated after incubation with 560 mu g/ml of PLGA-ALE NPs; a significant increase was observed at the highest dilutions. The viability of human umbilical vein endothelial cells and bone marrow stromal cells (BMSC), evaluated through the neutral red test, was not affected by PLGA-ALE NPs. There were no significant differences in cell-associated alkaline phosphatase between BMSC incubated with PLGA-ALE NP- and PBS-added media. These results demonstrated that PLGA-ALE NPs had an acceptable degree of blood compatibility and were not cytotoxic; therefore, they may be considered suitable for intravenous administration. (c) 2007 Elsevier Ltd. All rights reserved.