Nucleotide sequence of katF of Escherichia coli suggests KatF protein is a novel sigma transcription factor.
Nucleotide sequence of katF of Escherichia coli suggests KatF protein is a novel sigma transcription factor.
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大肠杆菌katF的核苷酸序列表明KatF蛋白是一种新型的σ转录因子。
DOI：
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发表时间：
1989
影响因子：
14.9
通讯作者：
Peter C. Loewen
中科院分区：
文献类型：
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作者：
M. Mulvey;Peter C. Loewen
对大肠杆菌katF基因进行了测序，揭示了一个1086个碱基对的开放阅读框架，从该框架中推导出了362个氨基酸的蛋白质序列。katF的转录方向通过在T7启动子后面双向克隆的基因的表达来证实。在体外表达的KatF蛋白迁移的表观大小为42 kDa。katF序列与编码RNA聚合酶的sigma亚基的rpoD序列的比较揭示了具有65%同源性的181 bp区域和具有87%同源性的38 bp片段。发现预测的KatF蛋白序列的62个氨基酸区域与σ 70的相应序列具有85%的同源性，包括涉及核心聚合酶结合的片段。与htpR编码的热休克调节蛋白也观察到同源性。
The katF gene of Escherichia coli has been sequenced revealing a 1086 base pair open reading frame from which the sequence of a 362 amino acid protein has been deduced. The direction of transcription of katF was confirmed by expression of the gene cloned in both directions behind a T7 promoter. The KatF protein expressed in vitro migrates with an apparent size of 42 kDa. Comparison of the katF sequence to the sequence of rpoD, which encodes the sigma subunit of RNA polymerase, revealed a 181 bp region with 65% homology and a 38 bp segment that was 87% homologous. A 62 amino acid region of the predicted KatF protein sequence was found to be 85% homologous to the corresponding sequence of sigma 70, including a segment implicated in core polymerase binding. Homology was also observed with the heat shock regulatory protein encoded by htpR.