Isolation of extracellular nanovesicle microRNA from liver cancer cells in culture.
Isolation of extracellular nanovesicle microRNA from liver cancer cells in culture.
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DOI：
10.1007/978-1-62703-453-1_2
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Patel, Tushar
中科院分区：
文献类型：
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作者：
Kogure, Takayuki;Patel, Tushar
MicroRNA可以在细胞外囊泡（如外泌体）内跨细胞转移。为了分析细胞外囊泡microRNA的生物学作用，有必要分离这些囊泡并提取其microRNA含量。在这里，我们描述了一种从肝癌细胞系中分离细胞纳米囊泡的方法，该方法可用于分离RNA和microRNA。
MicroRNA can be transferred across cells within extracellular vesicles such as exosomes. In order to analyze the biological effects of extracellular vesicle microRNA, it is necessary to isolate these vesicles and to extract their miRNA content.Here, we describe an approach to the isolation of cellular nanovesicles from liver cancer cell lines that can be used for the isolation of RNA and microRNA.