A further step in the evolution of rabbit hemorrhagic disease virus: the appearance of the first consistent antigenic variant
A further step in the evolution of rabbit hemorrhagic disease virus: the appearance of the first consistent antigenic variant
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DOI：
10.1016/s0168-1702(98)00106-3
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发表时间：
1998-11-01
期刊：
影响因子：
5
通讯作者：
Brocchi, E
中科院分区：
文献类型：
--
作者：
Capucci, L;Fallacara, F;Brocchi, E
兔出血症病毒（Rabbit Hemorrhagic Disease Virus，RHDV）是一种不可培养的杯状病毒，感染家兔引起急性致死性肝炎。1997年，我们从地理上遥远的意大利地区鉴定了两个RHDV分离株，其抗原性不同于参考株RHDV.Bs89。事实上，它们与mAb 1H 8不反应，mAb 1H 8能够保护兔免受RHD，并且与针对RHDV.Bs89产生的兔恢复期血清显示低反应性。兔与RHDV分离株的实验感染证实了它们的高致病性和它们特有的抗原谱;然而，用目前的疫苗接种的兔被保护免受这些分离株的攻击感染。序列比较表明，这两株病毒来源于同一株RHDV变异株，其结构蛋白（VP 60）序列与RHDV.Bs89的相似性为93%。该变体被命名为RHDVa，因为其与野生型RHDV显示出一致的遗传和抗原差异。特别地，RHDVa VP 60中44%的氨基酸取代位于氨基酸344和370之间，其中与RHDV.Bs89的相似性下降至70%，表明该区域可能含有mAb 1H 8识别的表位。此外，本文还对该病毒的血凝位点VP 60的氨基酸序列进行了初步分析。(C)1998年，Elsevier Science B. V.版权所有。
Rabbit hemorrhagic disease virus (RHDV) is a noncultivable calicivirus that infects rabbits (Oryctolagus cuniculus) and causes epidemics of an acute fatal hepatitis. In 1997 we identified two RHDV isolates from geographically distant Italian regions, which differed antigenically from the reference strain RHDV.Bs89. In fact, they were not reactive with mAb 1H8, that is able to protect rabbits from RHD and showed a low reactivity with the rabbit convalescent serum raised against RHDV.Bs89. Experimental infection of rabbits with either RHDV isolates confirmed their high pathogenicity and their peculiar antigenic profile; nevertheless, rabbits vaccinated with the current vaccine were protected against challenge infection with these isolates. Sequence comparison definitely demonstrated that the two isolates originated from the same RHDV variant and that the similarity of their structural protein (VP60) sequences with the RHDV.Bs89 is equal to 93%. This variant was named RHDVa since shows consistent genetic and antigenic differences from the wild-type RHDV. In particular, 44% of amino acid substitutions in RHDVa VP60 were located between amino acids 344 and 370, where the similarity with RHDV.Bs89 drops to 70%, suggesting that this region probably contains the epitope recognized by mAb 1H8. In addition: this paper presents preliminary data concerning the amino acids of VP60 involved in the hemagglutination site of the virus. (C) 1998 Elsevier Science B.V. All rights resented.