INTER-CELLULAR COMMUNICATION IN PANCREATIC-ISLET MONOLAYER-CULTURES - MICRO-FLUOROMETRIC STUDY
INTER-CELLULAR COMMUNICATION IN PANCREATIC-ISLET MONOLAYER-CULTURES - MICRO-FLUOROMETRIC STUDY
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DOI：
10.1126/science.35828
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发表时间：
1979-01-01
期刊：
影响因子：
56.9
通讯作者：
RABINOVITCH, A
中科院分区：
文献类型：
--
作者：
KOHEN, E;KOHEN, C;RABINOVITCH, A
将荧光素显微注射到新生大鼠胰腺单层培养物中的单个胰岛细胞，并通过显微荧光法进行地形扫描。荧光素从注射的胰岛细胞直接扩散到邻近的胰岛细胞中，并且在存在 16.7 mM 葡萄糖的情况下，与无葡萄糖培养基中相比，与注射的细胞通讯的胰岛细胞明显更多。胰岛细胞还被显微注射了糖酵解底物和激活剂，使还原吡啶核苷酸 -NADH 和 NADPH 的细胞水平产生短暂变化。首先在非常低的葡萄糖浓度下孵育 18 小时，然后在无葡萄糖培养基中并注射糖酵解底物和激活剂，在胰岛细胞中观察到 NADPH 荧光的变化；然而，相邻胰岛细胞的荧光几乎没有变化。相反，在培养基中添加 16.7 mM 葡萄糖后，注射糖酵解底物和激活剂会在注射的细胞和邻近细胞中产生 NADPH 荧光的短暂变化。
Single islet cells in monolayer cultures of neonatal rat pancreas were microinjected with fluorescein and scanned topographically by microfluorometry. Fluorescein spread from an injected islet cell directly into neighboring islet cells, and, in the presence of 16.7 mM glucose, significantly more islet cells communicated with the injected cell than in glucose-free medium. Islet cells were also microinjected with glycolytic substrates and activators that produced transient changes in cellular levels of reduced pyridine nucleotides-NADH and NADPH. Changes in NADPH fluorescence were observed in islet cells incubated first for 18 h in very low glucose concentrations and then in a glucose-free medium and injected with glycolytic substrates and activators; however, little change of fluorescence occurred in adjacent islet cells. In contrast, after adding 16.7 mM glucose to the medium, injection of glycolytic substrates and activators produced transient changes in NADPH fluorescence in the injected cell and in neighboring cells.