High-affinity alpha-thrombin binding to platelet glycoprotein Ib alpha: identification of two binding domains.
High-affinity alpha-thrombin binding to platelet glycoprotein Ib alpha: identification of two binding domains.
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高亲和力 α-凝血酶与血小板糖蛋白 Ib α 的结合：两个结合域的鉴定。
DOI：
10.1073/pnas.91.14.6334
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发表时间：
1994
影响因子：
11.1
通讯作者：
Krutzsch,H
中科院分区：
文献类型：
--
作者：
Gralnick,HR;Williams,S;McKeown,LP;Hansmann,K;Fenton2nd,JW;Krutzsch,H
α-凝血酶与血小板的结合和血小板的活化在生理性血栓的起始和动脉血栓形成的发生中是非常重要的。我们研究了凝血酶与人血小板结合的位点和亲和力。我们对肽抑制凝血酶结合的研究表明，糖蛋白Ib α结合位点具有高亲和力，Kd约为10（-10）M，而七跨膜结构域位点是中等亲和力的凝血酶结合位点，Kd约为10（-8）M。调节高亲和力或中等亲和力凝血酶结合的进一步研究可针对特定类别的位点。这将允许在不同的临床环境中部分或完全抑制特异性凝血酶-血小板相互作用。
alpha-Thrombin binding to and activation of platelets are of major importance in the initiation of physiologic thrombi and in the genesis of arterial thrombus formation. We have studied the site(s) and affinity of thrombin binding to human platelets. Our studies of the peptide inhibition of thrombin binding indicate that the glycoprotein Ib alpha binding site is of high affinity, Kd approximately 10(-10) M, while the seven-transmembrane-domain site is a moderate-affinity thrombin binding site, Kd approximately 10(-8) M. Further studies to modulate the high- or moderate-affinity thrombin binding can be directed to a specific class of sites. This would allow partial or total inhibition of specific thrombin-platelet interaction(s) in different clinical settings.