Metagenome and Metatranscriptome Profiling of Moderate and Severe COPD Sputum in Taiwanese Han Males.
Metagenome and Metatranscriptome Profiling of Moderate and Severe COPD Sputum in Taiwanese Han Males.
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DOI：
10.1371/journal.pone.0159066
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Weng JT
中科院分区：
文献类型：
--
作者：
Lee SW;Kuan CS;Wu LS;Weng JT
慢性阻塞性肺疾病（COPD）是一种以进行性气流阻塞为特征的炎性肺病，目前是世界上第四大死亡原因。COPD的发病机制被认为涉及细菌感染和炎症。由于测序技术的进步，支持肺部微生物组与COPD之间存在关联的证据正在出现。然而，很少有研究探讨COPD肺部细菌群落的表达谱。在这项研究中，我们使用下一代测序技术在DNA和RNA水平上分析了4名中度和4名重度COPD男性患者的痰微生物组。我们发现，通过16S rRNA基因测序确定的细菌组成可能不会直接翻译为转录组测序定义的活性表达细菌。两个测序数据一致认为普雷沃氏菌属、罗氏菌属、奈瑟氏菌属、卟啉单胞菌属、韦荣氏菌属、梭杆菌属和链球菌属是中度和重度COPD样本之间丰度差异最大的属，支持它们与COPD严重程度的相关性。然而，两个测序分析不同意这些细菌在两个COPD组中的相对丰度，这意味着研究活跃表达的细菌对于丰富我们对COPD的理解的重要性。虽然我们已经描述了中度和重度COPD中肺部微生物组的元转录组谱，但需要进一步研究以确定肺部微生物物种与COPD发病机制之间关系的功能基础。
Chronic obstructive pulmonary disease (COPD) is an inflammatory lung disorder characterized by the progressive obstruction of airflow and is currently the fourth leading cause of death in the world. The pathogenesis of COPD is thought to involve bacterial infections and inflammations. Owing to advancement in sequencing technology, evidence is emerging that supports an association between the lung microbiome and COPD. However, few studies have looked into the expression profile of the bacterial communities in the COPD lungs. In this study, we analyzed the sputum microbiome of four moderate and four severe COPD male patients both at the DNA and RNA level, using next generation sequencing technology. We found that bacterial composition determined by 16S rRNA gene sequencing may not directly translate to the set of actively expressing bacteria as defined by transcriptome sequencing. The two sequencing data agreed on Prevotella, Rothia, Neisseria, Porphyromonas, Veillonella, Fusobacterium and Streptococcus being among the most differentially abundant genera between the moderate and severe COPD samples, supporting their association with COPD severity. However, the two sequencing analyses disagreed on the relative abundance of these bacteria in the two COPD groups, implicating the importance of studying the actively expressing bacteria for enriching our understanding of COPD. Though we have described the metatranscriptome profiles of the lung microbiome in moderate and severe COPD, further investigations are required to determine the functional basis underlying the relationship between the microbial species in the lungs and pathogenesis of COPD.