Dynamics and Diversity of Escherichia coli in Animals and System Management of the Manure on a Commercial Farrow-to-Finish Pig Farm
Dynamics and Diversity of Escherichia coli in Animals and System Management of the Manure on a Commercial Farrow-to-Finish Pig Farm
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DOI：
10.1128/aem.02866-12
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发表时间：
2013-02-01
影响因子：
4.4
通讯作者：
Moreno, Miguel A.
中科院分区：
文献类型：
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作者：
Marchant, Mauricio;Moreno, Miguel A.
本研究的目的是确定西班牙一家商业产仔猪场在整个生产周期（2008 年 7 月至 2009 年 7 月）的动物和环境品系中大肠杆菌种群的动态和多样性，特别关注抗菌素耐药性和整合子的存在。在动物系中，从怀孕母猪（10个样品和20个分离株）、1周龄仔猪（20个样品和40个分离株）、未断奶仔猪（20个样品和38个分离株）、饲养猪（20个样品和40个分离株）和育肥猪舍（6个样品和118个分离株）中收集了总共256个分离株；从地下坑和农场泥浆罐环境线中，分别收集到 100 和 119 株分离株。我们的结果表明，猪粪便微生物群和农场环境中的大肠杆菌种群具有高度动态性并表现出高度的多样性。这些问题已通过基于 DNA 的分型数据（重复外基因回文 PCR [REP-PCR]）和表型分型数据（包含 19 种抗菌药物的抗菌药物耐药性概况）得到证实。基于REP-PCR分型结果对采样组进行聚类表明，对于大肠杆菌群体的聚类，空间特征（线）比时间特征（采样周）具有更强的权重；当基于抗性类型进行聚类时，该权重不太显着。考虑到 REP-PCR 指纹和耐药型，在动物中，育肥猪体内的大肠杆菌群与所研究的其余动物群不同。从公共卫生角度来看，这一人群是最重要的，其抗菌素耐药性和整合子的存在水平最低。
The objective of this study was to determine the dynamics and diversity of Escherichia coli populations in animal and environmental lines of a commercial farrow-to-finish pig farm in Spain along a full production cycle (July 2008 to July 2009), with special attention to antimicrobial resistance and the presence of integrons. In the animal line, a total of 256 isolates were collected from pregnant sows (10 samples and 20 isolates), 1-week-old piglets (20 samples and 40 isolates), unweaned piglets (20 samples and 38 isolates), growers (20 samples and 40 isolates), and the finishers' floor pen (6 samples and 118 isolates); from the underfloor pits and farm slurry tank environmental lines, 100 and 119 isolates, respectively, were collected. Our results showed that E. coli populations in the pig fecal microbiota and in the farm environment are highly dynamic and show high levels of diversity. These issues have been proven through DNA-based typing data (repetitive extragenic palindromic PCR [REP-PCR]) and phenotypic typing data (antimicrobial resistance profile comprising 19 antimicrobials). Clustering of the sampling groups based on their REP-PCR typing results showed that the spatial features (the line) had a stronger weight than the temporal features (sampling week) for the clustering of E. coli populations; this weight was less significant when clustering was performed based on resistotypes. Among animals, finishers harbored an E. coli population different from those of the remaining animal populations studied, considering REP-PCR fingerprints and resistotypes. This population, the most important from a public health perspective, demonstrated the lowest levels of antimicrobial resistance and integron presence.