Adventitious shoot regeneration in cotyledons from Japanese pear and related species
Adventitious shoot regeneration in cotyledons from Japanese pear and related species
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DOI：
10.1007/s11627-012-9451-2
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发表时间：
2012-08-01
影响因子：
2.6
通讯作者：
Yamamoto, Toshiya
中科院分区：
文献类型：
--
作者：
Nakajima, Ikuko;Ito, Akiko;Yamamoto, Toshiya
以日本梨及其近缘种的子叶为外植体，建立了一种高效的不定芽诱导方法。以日本梨“Okusankichi”和亚洲豌豆梨“Hokushimamenashi”种子的子叶为材料，研究了培养基中植物激素的最适浓度。当培养基中含有5 μ M的1-萘乙酸和10或25 μ M的6-苄氨基嘌呤时，不定芽的产生率和每个外植体的不定芽数最高。这些生长调节剂被用来产生不定芽从子叶的33个品种的日本，中国，亚洲豌豆，和欧洲梨。以日本梨“今村明”和“阿根昭信梨”子叶为外植体，获得了较高的不定芽数（1.3-2.3个）和不定芽再生率（60- 76%）。虽然授粉不受控制，但来自日本老品种和中国梨母树的子叶往往比来自其他梨品种的子叶更容易再生。由于不定芽生根能力很低，采用微嫁接技术获得了不定芽再生植株。嫁接再生植株的回收率超过60%，无论品种。据我们所知，这是第一份报告，以评估芽再生子叶的主要梨品种。
An efficient method for the generation of adventitious shoots from the cotyledons of Japanese pear and related species was developed. Cotyledons from seeds of the Japanese pear "Okusankichi" and the Asian pea pear "Hokushimamenashi" were used to determine the optimum concentrations of phytohormones in the medium. The rates of generation of adventitious shoots and the numbers of adventitious shoots per explant were highest when the media contained 5 mu M 1-naphthaleneacetic acid combined with 10 or 25 mu M 6-benzylaminopurine. These growth regulators were used to generate adventitious shoots from the cotyledons of 33 cultivars of Japanese, Chinese, Asian pea, and European pears. A high number of adventitious shoots per explant (1.3-2.3) and high rates of regeneration of adventitious shoots (60-76 %) were obtained from the cotyledons of Japanese pears "Imamuraaki" and "Agenosho Shinanashi." Although pollination was not controlled, cotyledons from mother trees of old Japanese cultivars and Chinese pears tended to be more regenerable than those from other pear species. Since the rooting ability of the adventitious shoots was very low, micro-grafting was applied to obtain regenerated potted plants from adventitious shoots. Grafted regenerated plants were recovered at a rate of more than 60 %, regardless of cultivar. To our knowledge, this is the first report to evaluate shoot regeneration from cotyledons of major Pyrus species.