Amino acid analysis using core-shell particle column
Amino acid analysis using core-shell particle column
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DOI：
10.1016/j.jchromb.2012.09.005
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发表时间：
2013-05-15
影响因子：
3
通讯作者：
Tsunoda, Makoto
中科院分区：
文献类型：
--
作者：
Song, Yanting;Funatsu, Takashi;Tsunoda, Makoto
在这项研究中，核壳颗粒柱的分离效率进行了比较，颗粒填充和整体硅胶柱，这表明，核壳颗粒柱具有较小的理论塔板高度，其分离效率不受流量的增加显着。建立了一种高效液相色谱法测定氨基酸的新方法。以4-氟-7-硝基-2，1，3-苯并恶二唑（NBD-F）为荧光衍生试剂，在Kinetex C18柱上进行分离。21种NBD-氨基酸的分析时间在7 min内，比我们以前的研究与传统的颗粒填充柱或整体硅胶柱快。所有氨基酸的校准曲线的线性度被认为是良好的注射量范围从40 fmol到40 pmol。氨基酸测定的准确度为90.9- 107%。该方法可用于生物样品中氨基酸的快速测定。(C)2012爱思唯尔有限公司版权所有。
In this study, the separation efficiency of a core-shell particle column was compared with particle-packed and monolithic silica columns, which showed that the core-shell particle column had a smaller theoretical plate height and that its separation efficiency was not affected significantly by the increase in flow rate. A fast HPLC method using a core-shell particle column was developed for the determination of amino acids. 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was used as a fluorescence derivatization reagent for amino acids, followed by separation on a core-shell Kinetex C18 column. The analysis time for 21 NBD-amino acids was within 7 min, which was faster than that in our previous studies with conventional particle-packed columns or monolithic silica columns. The linearities of the calibration curves for all the amino acids were found to be good over a range of injection amounts from 40 fmol to 40 pmol. The accuracies for the amino acid determinations were 90.9-107%. The method was proved to have potential for the fast determination of amino acids in biological samples. (C) 2012 Elsevier B.V. All rights reserved.