Polygonibacillus indicireducens gen. nov., sp. nov., an indigo-reducing and obligate alkaliphile isolated from indigo fermentation liquor for dyeing
Polygonibacillus indicireducens gen. nov., sp. nov., an indigo-reducing and obligate alkaliphile isolated from indigo fermentation liquor for dyeing
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DOI：
10.1099/ijsem.0.001405
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发表时间：
2016-11-01
影响因子：
2.8
通讯作者：
Yumoto, Isao
中科院分区：
文献类型：
--
作者：
Hirota, Kikue;Okamoto, Takahiro;Yumoto, Isao
从发酵的靛蓝蓼（Polygonum tinctorium Lour.）从日本北海道伊达市的工艺中心获得的酒样品。16 S rRNA基因序列同源性表明菌株In 2 -9（T）是芽孢杆菌属的成员，与芽孢杆菌属的嗜碱物种，解半纤维素芽孢杆菌JCM 9152（T）（96.4% 16 S rRNA基因序列相似性）和嗜碱芽孢杆菌DSM 485（T）（96.5%）最接近。分离物的细胞染色为革兰氏阳性，为兼性厌氧直杆，通过周毛鞭毛运动。菌株In 2 -9（T）在13 - 45 ℃之间生长，最佳生长在约35-37 ℃。菌株在pH 8-12范围内生长，最适生长pH 10。检测到的类异戊二烯醌是甲基萘醌-6（MK-6），DNA G+C含量为39.4mol%。全细胞脂肪酸谱主要（> 10%）由异-C-15：0、反异-C-15：0和C-16：0组成。孢子的形状和位置和化学分类学特征表明，菌株是明显不同的系统发育相邻的嗜碱芽孢杆菌属物种。基于表型和化学分类学特征以及系统发育数据，分离物代表了一个新属的新种，其名称为Polygonibacillus indicirecucens gen. nov.，sp. nov.的报道。模式种的模式菌株是In 2 -9（T）（= JCM 30831（T）= NCIMB 14982（T）），菌株D2-7是该种的附加菌株。
Obligately alkaliphilic and halophilic strains, designated In2-9(T) and D2-7, were isolated from a fermented Polygonum indigo (Polygonum tinctorium Lour.) liquor sample obtained from a craft centre in Date City, Hokkaido, Japan. The 16S rRNA gene sequence phylogeny suggested that strain In2-9(T) is a member of the genus Bacillus with the closest relatives being the alkaliphilic species of the genus Bacillus, Bacillus hemicellulosilyticusJCM 9152(T) (96.4% 16S rRNA gene sequence similarity) and Bacillus alcalophilus DSM 485(T) (96.5 %). Cells of the isolate stained Gram-positive and were facultatively anaerobic straight rods that were motile by peritrichous flagella. Strain In2-9(T) grew between 13 and 45 degrees C with optimum growth at approximately 35-37 degrees C. The isolates grew in the pH range of 8-12 with optimum growth at pH 10. The isoprenoid quinone detected was menaquinone-6 (MK-6) and the DNA G+C content was 39.4 mol%. The whole-cell fatty acid profile mainly (> 10 %) consisted of iso-C-15 : 0, anteiso-C-15 : 0 and C-16 : 0. Spore shape and location and chemotaxonomic characteristics revealed that the isolates were distinctly different from phylogenetic neighbouring alkaliphilic species of the genus Bacillus. On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, the isolates represent a novel species of a new genus, for which the name Polygonibacillus indicireducens gen. nov., sp. nov. is proposed. The type strain of the type species is In2-9(T) (= JCM 30831(T) = NCIMB 14982(T)), and strain D2-7 is an additional strain of the species.