The genetic transformation of Pythium guiyangense mediated by Agrobacterium tumefaciens
The genetic transformation of Pythium guiyangense mediated by Agrobacterium tumefaciens
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根癌农杆菌介导贵阳腐霉的遗传转化
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发表时间：
2008
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中科院分区：
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贵阳腐霉（Pythium guiyangense）是一种重要的蚊虫病原菌，是一种很有前途的生物防治剂。为了获得适应不同生态环境、对蚊幼虫毒力稳定、货架期延长的菌株，本研究利用同源或异源毒力基因对该真菌进行了转化。本文报道了根癌农杆菌介导的贵阳松遗传转化试验。因此，A.成功建立了根癌农杆菌介导的遗传转化体系。
Pythium guiyangense is a mosquito pathogen, and has been proved to be a promising agent for biological control of mosquitoes. In order to develop the strains adaptable to different ecological environment having stable virulence to mosquito larvae, and being able to prolong the shelf life, an effort was made on transforming the fungus by using homologous or heterologous virulence genes. In this paper, a genetic transformation experiment of P. guiyangense mediated by Agrobacterium tumefaciens is reported. As a result, an A. tumefaciens mediated genetic transformation system was established successfully.