H2B (Ser10) phosphorylation is induced during apoptosis and meiosis in S-cerevisiae
H2B (Ser10) phosphorylation is induced during apoptosis and meiosis in S-cerevisiae
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DOI：
10.4161/cc.4.6.1745
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发表时间：
2005-06-01
期刊：
影响因子：
4.3
通讯作者：
Allis, CD
中科院分区：
文献类型：
--
作者：
Ahn, SH;Henderson, KA;Allis, CD
核小体由高度保守的组蛋白八聚体和相关的DNA组成，是真核染色质的基本单位。核小体阵列是如何折叠成高阶结构的，以及这种压缩的动力学是如何调节的，这些问题在很大程度上仍然没有答案。我们最近的研究表明，在酵母（丝氨酸10）(1)和哺乳动物细胞（丝氨酸14）中，组蛋白H2B的磷酸化是诱导细胞死亡的必要条件，细胞死亡表现出凋亡的表型特征，包括DNA断裂和染色质凝聚。(2)在这篇文章中，我们通过揭示H2B丝氨酸10 （Ser10）磷酸化在另一个生物学事件中的作用来扩展这些发现，该事件与高阶染色质结构减数分裂的显著改变有关。我们的数据显示，在酵母减数分裂前期的粗线期，强烈的染色表明H2B （Ser10）磷酸化。这些数据拓宽了磷酸化标记在染色质重塑中的应用，它与染色质压实密切相关。磷酸化标记如何在细胞凋亡和减数分裂等多种过程中转化为有意义的下游事件仍然是未来研究的挑战。
The nucleosome, composed of an octamer of highly conserved histone proteins and associated DNA, is the fundamental unit of eukaryotic chromatin. How arrays of nucleosomes are folded into higher-order structures, and how the dynamics of such compaction are regulated, are questions that remain largely unanswered. Our recent studies demonstrated that phosphorylation of histone H2B is necessary to induce cell death that exhibits phenotypic hallmarks of apoptosis including DNA fragmentation and chromatin condensation in yeast ( serine 10)(1) and in mammalian cells ( serine 14).(2) In this article, we extend these findings by uncovering a role for H2B phosphorylation at serine 10 (Ser10) in another biological event that is associated with dramatic alterations in higher-order chromatin structure, meiosis. Our data show strong staining, indicative of H2B ( Ser10) phosphorylation, during the pachytene stage of yeast meiotic prophase. These data broaden the use of this phosphorylation mark in chromatin remodeling that closely correlates with chromatin compaction. How phosphorylation marks are translated into meaningful downstream events during processes as diverse as apoptosis and meiosis remain a challenge for future studies.