Framework YAC contig anchored into a 3.2-Mb high-resolution physical map in proximal 11q13.
Framework YAC contig anchored into a 3.2-Mb high-resolution physical map in proximal 11q13.
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框架 YAC 重叠群锚定到近端 11q13 的 3.2 Mb 高分辨率物理图谱中。
DOI：
10.1006/geno.1996.4527
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发表时间：
1997
期刊：
影响因子：
4.4
通讯作者：
Gaudray,P
中科院分区：
文献类型：
--
作者：
Courseaux,A;Szepetowski,P;Fernandes,M;Serizet,C;Kawaguchi,Y;Grosgeorge,J;Perucca-Lostanlen,D;Shows,TB;Todd,JA;Nowak,NJ;Gaudray,P
尽管在11号染色体q13带上存在许多使个体易患各种人类疾病的基因，但该基因组区域的大部分仍然是松散的。此外，酵母人工染色体（YAC）在全基因组研究中相对缺乏：YAC不规则地分布在该染色体区域，并没有排列成contigs。因此，我们对actn3和fgf3两侧的3.2 mb区域进行了精细测绘。由于该区域表现出高度的YAC不稳定性，我们建立了一个框架，通过将YAC和cosmids锚定到基于荧光原位杂交和远程限制图谱的高分辨率物理图谱中。研究的3.2 mb区域包括被认为含有使个体易患骨质疏松-假性胶质瘤综合征和胰岛素依赖性糖尿病的基因的区域边界，以及驱动人类癌症扩增事件的基因。这个基因组区域的另一个特征是它与基因组的非同染色体区域交叉杂交。此外，它跨越了与小鼠第19号染色体共体保护结束的区域，使我们在那里固定的克隆成为理解基因组进化的宝贵工具。
Despite the presence on band q13 of chromosome 11 of a number of genes predisposing individuals to various human diseases, most of this genomic region remains loosely mapped. Moreover, there is a relative dearth of yeast artificial chromosome (YAC) contigs from genome-wide studies: YACs are irregularly distributed over this chromosomal region and have not been arranged into contigs. We have thus undertaken fine-scale mapping of a 3.2-Mb region flanked byACTN3andFGF3.Since this region has demonstrated a high degree of YAC instability, we have established a framework contig by anchoring YACs and cosmids into a high-resolution physical map based on fluorescencein situhybridization and long-range restriction mapping. The 3.2-Mb area studied includes the boundaries of regions thought to contain genes predisposing individuals to osteoporosis–pseudoglioma syndrome and insulin-dependent diabetes mellitus, as well as genes driving amplification events in human carcinomas. Another feature of this genomic area is that it cross-hybridizes to nonsyntenic regions of the genome. In addition, it spans the region where syntenic conservation with mouse chromosome 19 ends, making clones that we have anchored there valuable tools in understanding genome evolution.