Flow linear dichroism and Fourier transform IR spectra reveal geometry for X-form DNA.
Flow linear dichroism and Fourier transform IR spectra reveal geometry for X-form DNA.
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流动线性二色性和傅里叶变换红外光谱揭示了 X 型 DNA 的几何形状。
DOI：
10.1002/bip.360341104
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发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Vorlickova,M
中科院分区：
文献类型：
--
作者：
Riazance-Lawrence,JH;Kang,H;Chou,PJ;JohnsonJr,WC;Vorlickova,M
流动线性二色性测量延伸到真空紫外区，得到X形聚(DADT)·聚(DADT)的基本法线从螺旋轴21°(Da)和40°(DT)的倾角。这些倾斜角类似于合成聚合物的B型，但碱基倾斜的轴线不同。氢键碱基对与天然DNA标准B、C、D和Z型的几何构型一致，但不适合A型。傅立叶变换红外光谱表明，X-型具有类似于B-型DNA的糖折叠和磷酸构型，并支持与早期工作中看到的两种磷酸盐的二核苷酸重复，类似于Z-型DNA。显然，X-Form DNA有一个独特的几何结构。©1994 John Wiley&Sons，Inc.
Flow linear dichroism measurements extended into the vacuum uv region yield inclinations for the base normal from the helix axis of 21° for dA and 40° for dT in the X‐form of poly (dAdT) · poly (dAdT). These inclination angles are similar to the B form of the synthetic polymer, but the axes around which the bases incline are different. Hydrogen‐bonded base pairs are consistent with the geometry for the standard B, C, D, and Z forms of natural DNA, but will not fit into the A form. Fourier transform ir spectra indicate that the X form has sugar pucker and phosphate geometry similar to B‐form DNA, and supports the dinucleotide repeat with two kinds of phosphates seen in earlier work, in analogy to Z‐form DNA. Clearly, X‐form DNA has a unique geometry. © 1994 John Wiley & Sons, Inc.