Investigation of liposome-based immunomigration sensors for the detection of polychlorinated biphenyls.
Investigation of liposome-based immunomigration sensors for the detection of polychlorinated biphenyls.
复制标题
用于检测多氯联苯的基于脂质体的免疫迁移传感器的研究。
DOI：
10.1021/ac00099a002
复制
发表时间：
1995
影响因子：
7.4
通讯作者：
Durst,RA
中科院分区：
文献类型：
--
作者：
Roberts,MA;Durst,RA
使用免疫特异性脂质体迁移为实验室外环境污染物的检测提供了许多优势。利用该技术的设备快速，易于使用，坚固耐用，并对低十亿分之一浓度的分析物做出反应。已经开展了调查，以确定这些测定的关键组分的最佳相互作用，并提出了开发广义脂质体免疫测定的技术。开发了两种互补的基于脂质体的免疫迁移技术原型，用于检测多氯联苯（pcb）。脂质体免疫竞争检测格式测量分析物标记的脂质体与固定抗体的分析物之间的竞争反应，可以在不到8min的时间内检测0.4 nmol的PCB。脂质体免疫聚集（liposome immunoaggregation， LIA）是一种更灵敏的方法，可以检测到溶液中免疫特异性脂质体聚集的抑制作用，并且可以在不到23分钟的时间内检测到2.6 pmol的PCB。激光衍射粒度已用于研究lia诱导的脂质体尺寸随时间的增加，并确定该技术应用的最佳条件。这两种形式都利用毛细管作用沿硝酸纤维素条输送含脂质体的溶液。然后用视觉或桌面扫描仪测量颜色强度。多氯联苯（PCBs）在美国以Aroclor商标进行商业销售，是一种工业化合物，广泛用作润滑剂、阻燃剂、浸没油、介质和传热流体，以及许多其他产品。它们污染了各种各样的介质，主要是由于粗心的使用、处置和事故，现在已被EPA确定为国家超级基金计划下优先整治的污染物。最近，环境保护署和各种环境补救公司进行了广泛的努力，以确定超级基金地点的特征。迅速完成这类研究的主要障碍之一是由非现场实验室进行传统的多氯联苯分析的高成本和低周转时间。
The use of immunospecific liposome migration offers many advantages for the extralaboratory detection of environmental contaminants. Devices utilizing this tech-nique are fast, easy to use, and robust and respond to the presence of analyte at low-parts-per-billion concentra-tions. Investigations have been carried out that determine optimal interactions for key components of these assays, and techniques are presented for the development of generalized liposome immunoassays. Two complementary prototype liposome-based immunomigration tech-niques have been developed for the detection of polychlo-rinated biphenyls (PCBs). The liposome immunocompetition assay format measures the competitive reac-tion between analyte-tagged liposomes and the sample analyte for immobilizedantibodies and can detect 0.4 nmol of PCB in less than 8min. A more sensitive format, the liposome immunoaggregation (LIA) assay detects the inhibition of immunospecificliposome aggregation in solution and can detect 2.6 pmol of PCB in less than23 min. Laser diffraction particle sizing has been used to study LIA-induced increases in liposome size over time and to determine optimal conditions for the application of this technique. Both formats utilize capillary action to transport liposome-containing solutions along strips of nitrocellulose. Measurement of color intensity is then carried out visually or with a desktop scanner.Polychlorinated biphenyls (PCBs), which were sold com-mercially in the United States under the Aroclor trademark, were industrial compounds used extensively as lubricants, fire retar-dants, immersion oils, and dielectric and heat transfer fluids, as well as a multitude of other products. 1 They have contaminated an enormous variety of media, primarily as a result of careless use, disposal, and accidents and have now been identified by the EPA as priority pollutantsto be targeted for remediation under the national Superfund program. Extensive efforts have recently been undertaken to characterize Superfund sites by both the EPA and various environmental remediation firms. One of the chief obstacles to the prompt completion of such studies is the high cost and low turnaround time for conventionalPCB analysis by off-site laboratories.