INVITRO CONTROL OF BLASTEMA CELL-PROLIFERATION BY EXTRACTS FROM EPIDERMAL CAP AND MESENCHYME OF REGENERATING LIMBS OF AXOLOTLS
INVITRO CONTROL OF BLASTEMA CELL-PROLIFERATION BY EXTRACTS FROM EPIDERMAL CAP AND MESENCHYME OF REGENERATING LIMBS OF AXOLOTLS
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DOI：
10.1007/bf00399054
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发表时间：
1990-01-01
期刊：
影响因子：
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通讯作者：
ALBERT, P
中科院分区：
文献类型：
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作者：
BOILLY, B;ALBERT, P
在芽基粗提物中检测到蝾螈肢芽基中存在促有丝分裂活性。提取物的促有丝分裂性从芽基细胞的有丝分裂指数估计，芽基细胞在肢芽基提取物存在下生长6天，最后2天存在秋水仙素。所有测试的提取物（整个芽基，芽基间充质，表皮帽）显着增强芽基细胞的增殖。我们观察到的最高刺激因子为7 × 10 - 6。用7 μ g蛋白质/ml全芽基提取物，5.2 ×用14 μ g/ml胚根间充质提取物和11 x.用3.5 μ g/ml表皮帽提取物。因此，表皮帽提取物似乎是最具促有丝分裂性的。来自胚间充质的提取物，尽管比表皮帽提取物促有丝分裂性低，但比神经提取物更有效[Albert P，Boilly B（1986）Biol Cell 58：251-262]。这些结果进行了讨论，在肢体再生过程中的生长因子的生产。
The presence of a mitogenic activity in limb blastemas of axolotls was detected in crude extracts of blastemas at the mid-bud stage. The mitogenicity of the extracts was estimated from the mitotic index of blastema cells grown for 6 days in the prisence of limb blastema extracts, with colchicine present for the last 2 days. All the extracts tested (whole blastema, blastemal mesenchyme, epidermal cap) significantly enhanced proliferation of blastema cells. The highest stimulation factors we observed were 7 .times. with 7 .mu.g protein/ml whole blastema extracts, 5.2 .times. with 14 .mu.g/ml blastemal mesenchyme extracts, and 11 .times. with 3.5 .mu.g/ml epidermal cap extracts. Hence the epidermal cap extracts appeared to be the most mitogenic. Extracts from the blastemal mesenchyme, although less mitogenic than the epidermal cap extracts, were more potent than nerve extracts [Albert P, Boilly B (1986) Biol Cell 58:251-262]. These results are discussed with regard to the production of growth factors during limb regeneration.