Visualizing chemokine-dependent T cell activation and migration in response to central nervous system infection.
Visualizing chemokine-dependent T cell activation and migration in response to central nervous system infection.
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DOI：
10.1007/978-1-62703-426-5_11
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Wilson, Emma H
中科院分区：
文献类型：
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作者：
Carson, Monica J;Wilson, Emma H
响应于中枢神经系统（CNS）损伤和感染，星形胶质细胞、神经元和CNS脉管系统表达几种趋化因子，包括CCL21。定量聚合酶链反应（qPCR）、蛋白质印迹和免疫组织化学方法可以定量mRNA和蛋白质表达。然而，这些方法不能量化趋化因子的生物利用度和生物活性，这些变量受到许多环境因素的影响，包括细胞外基质（ECM）的组成。在这里，我们说明了如何双光子显微镜和羧基荧光素琥珀酰亚胺酯（CFSE或CFDA SE）标记的T细胞加上流式细胞术可以作为工具来评估趋化因子介导的调节T细胞增殖，活化和迁移。
In response to central nervous system (CNS) injury and infection, astrocytes, neurons, and CNS vasculature express several chemokines, including CCL21. Quantitative polymerase chain reaction (qPCR), western blot, and immunohistochemical methods can quantify mRNA and protein expression. However, these methods do not quantify chemokine bioavailability and bioactivity, variables modified by many environ mental factors including composition of extracellular matrix (ECM). Here we illustrate how two-photon microscopy and carboxyfluorescein succinimidyl ester (CFSE or CFDA SE) labeling of T cells coupled with flow cytometry can be used as tools to assess chemokine-mediated regulation of T cell proliferation, activation, and migration.