Effects of berberine on diabetes induced by alloxan and a high-fat/high-cholesterol diet in rats
Effects of berberine on diabetes induced by alloxan and a high-fat/high-cholesterol diet in rats
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DOI：
10.1016/j.jep.2006.04.019
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发表时间：
2006-11-03
影响因子：
5.4
通讯作者：
Liu, Sheng
中科院分区：
文献类型：
--
作者：
Tang, Li-Qin;Wei, Wei;Liu, Sheng
小檗碱是黄连的主要有效成分。本研究旨在探讨小檗碱对糖尿病大鼠的影响及其可能的机制。采用尾静脉注射四氧嘧啶诱导Wistar大鼠糖尿病。四氧嘧啶给药量为55 mg/kg。糖尿病大鼠喂食高胆固醇食物。采用分光光度法测定空腹血糖、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白-胆固醇（LDL-c）、高密度脂蛋白-胆固醇（HDL-c）、血清一氧化氮（NO）水平和心脏组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-px）含量。四氧嘧啶治疗3周后取胰腺标本，用苏木精-伊红（HE）染色，光镜下观察并评分。黄连素（100、200 mg/kg）灌胃可显著降低糖尿病大鼠空腹血糖水平，降低血清TC、TG、LDL-c含量，有效提高HDL-c、NO水平。此外，小檗碱处理明显阻断了MDA的增加。增加糖尿病大鼠的SOD和GSH-px水平。病理组织学结果显示，小檗碱能恢复糖尿病大鼠胰腺组织的损伤。结果表明，小檗碱能显著抑制四氧嘧啶诱导的糖尿病的进展，其对糖尿病的抑制作用可能与其降糖作用、调节脂质代谢作用和清除自由基能力有关。2006爱思唯尔爱尔兰有限公司版权所有。
Berberine is the major active constituent of Rhizoma coptidis. The present study was carried out to investigate the effect of berberine on diabetes in rats and its possible mechanisms. Diabetes was induced by tail vein injection with alloxan in Wistar rats. The amount of alloxan administered was 55 mg/kg. Diabetic rats were fed with a high-cholesterol diet. The fasting blood glucose, total cholesterol (TC), triglyceride (TG) and low density lipoprotein-cholesterol (LDL-c), high density lipoprotein-cholesterol (HDL-c), nitric oxide (NO) levels in serum and malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-px) contents in heart tissue were assayed by spectrophotometry. Pancreas samples collected after 3 weeks of alloxan treatment were stained with hematoxylin-eosin (HE) and examined under a light microscope, and scored. Intragastric administration of berberine (100 and 200 mg/kg) significantly decreased fasting blood glucose levels, serum content of TC, TG, LDL-c, and effectively increased HDL-c, NO level in diabetic rats. Furthermore, berberine treatment significantly blocked the increase of MDA,. increased SOD and GSH-px levels in diabetic rats. Histopathological scores showed that berberine had restored the damage of pancreas tissues in rats with diabetes mellitus. The results showed berberine significantly inhibited the progression of diabetes induced by alloxan, and the inhibitory effect of berberine on diabetes might be associated with its hypoglycemic effect, modulating lipids metabolic effects and its ability to scavenge free radical. (c) 2006 Elsevier Ireland Ltd. All rights reserved.