Discrimination of Pseudomonas syringae isolates from sweet and wild cherry using rep-PCR
Discrimination of Pseudomonas syringae isolates from sweet and wild cherry using rep-PCR
复制标题
使用rep-PCR 鉴别甜樱桃和野樱桃中的丁香假单胞菌分离株
DOI：
--
复制
发表时间：
2007
影响因子：
1.8
通讯作者：
S. J. Roberts
中科院分区：
文献类型：
--
作者：
J. Vicente;S. J. Roberts
细菌性溃疡病是樱桃的重要病害之一。该病害可由假单胞菌的两个致病变种引起：pv. morsprunorum和pv.你好研究了重复DNA聚合酶链反应指纹图谱（rep-PCR）技术在甜樱桃和野樱桃疫霉致病变种、生理小种和分离物鉴别中的应用。用REP（repeating extragenic palindromic）、ERIC（enterobacterial repeating intergenic consensus）和BOX引物对87株分离株的总基因组DNA进行扩增，然后进行琼脂糖凝胶电泳，各组分离株均显示特异性PCR产物。丁香假单胞菌金龟子分离株高度可变。结果表明，不同菌株的指纹图谱差异较大。morsprunorum小种1分离物较小。在此基础上，研究了烟草赤星病菌P. morsprunorum小种2分离物也是非常一致的（有一个例外），并且与小种1分离物不同。rep-PCR是一种快速、简便的方法，可用于鉴别两个小种的分离物。morsprunorum;该方法也可以辅助鉴定P.虽然它不能代替接种敏感的主机，如樱桃和丁香。
Bacterial canker is one of the most important diseases of cherry (Prunus avium). This disease can be caused by two pathovars of Pseudomonas syringae: pv. morsprunorum and pv. syringae. Repetitive DNA polymerase chain reaction-based fingerprinting (rep-PCR) was investigated as a method to distinguish pathovars, races and isolates of P. syringae from sweet and wild cherry. After amplification of total genomic DNA from 87 isolates using the REP (repetitive extragenic palindromic), ERIC (enterobacterial repetitive intergenic consensus) and BOX primers, followed by agarose gel electrophoresis, groups of isolates showed specific patterns of PCR products. Pseudomonas syringae pv. syringae isolates were highly variable. The differences amongst the fingerprints of P. syringae pv. morsprunorum race 1 isolates were small. The patterns of P. syringae pv. morsprunorum race 2 isolates were also very uniform, with one exception, and distinct from the race 1 isolates. rep-PCR is a rapid and simple method to identify isolates of the two races of P. syringae pv. morsprunorum; this method can also assist in the identification of P. syringae pv. syringae isolates, although it cannot replace inoculation on susceptible hosts such as cherry and lilac.