LOCAL-DELIVERY OF VASCULAR ENDOTHELIAL GROWTH-FACTOR ACCELERATES REENDOTHELIALIZATION AND ATTENUATES INTIMAL HYPERPLASIA IN BALLOON-INJURED RAT CAROTID-ARTERY
LOCAL-DELIVERY OF VASCULAR ENDOTHELIAL GROWTH-FACTOR ACCELERATES REENDOTHELIALIZATION AND ATTENUATES INTIMAL HYPERPLASIA IN BALLOON-INJURED RAT CAROTID-ARTERY
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DOI：
10.1161/01.cir.91.11.2793
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发表时间：
1995-06-01
期刊：
影响因子：
37.8
通讯作者：
ISNER, JM
中科院分区：
文献类型：
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作者：
ASAHARA, T;BAUTERS, C;ISNER, JM
背景大多数旨在通过使用药理学或生物试剂减少再狭窄的策略涉及直接抑制血管平滑肌细胞（SMC）增殖。或者，如果可以在球囊引起的动脉损伤部位特别促进再内皮化，则可能会间接抑制 SMC 增殖。因此，我们研究了这样的假设：将内皮细胞（EC）特异性有丝分裂原应用于新剥脱的内膜表面可以加速再内皮化，从而减弱内膜增生。 方法和结果对 31 只 Sprague-Dawley 大鼠的左颈动脉进行球囊损伤，然后对 16 只大鼠用 100 μg 血管内皮生长因子孵育 30 分钟。 (VEGF)，一种 EC 特异性有丝分裂原。对照动物 (n=15) 接受 0.9% 盐水孵育 30 分钟。球囊损伤后 2 周，VEGF 治疗组的颈动脉再内皮化明显优于对照组（14.59+/-1.12 对比 7.96+/-0.51 mm(2)，P
Background Most strategies designed to reduce restenosis by the use of pharmacological or biological reagents involve direct inhibition of vascular smooth muscle cell (SMC) proliferation. Alternatively, SMC proliferation might be indirectly inhibited if reendothelialization could be specifically facilitated at sites of balloon-induced arterial injury. Accordingly, we investigated the hypothesis that application of an endothelial cell (EC)-specific mitogen to a freshly denuded intimal surface could accelerate reendothelialization and thereby attenuate intimal hyperplasia.Methods and Results The left carotid artery of 31 Sprague-Dawley rats was subjected to balloon injury, after which 16 rats were treated with a 30-minute incubation with 100 mu g of vascular endothelial growth factor (VEGF), an EC-specific mitogen. Control animals (n=15) received a 30-minute incubation with 0.9% saline. At 2 weeks after balloon injury, carotid artery reendothelialization was markedly superior in the VEGF-treated group compared with the control group (14.59+/-1.12 versus 7.96+/-0.51 mm(2), P