Uptake of iopanoic acid by isolated rat hepatocytes in primary culture.
Uptake of iopanoic acid by isolated rat hepatocytes in primary culture.
复制标题
原代培养中分离的大鼠肝细胞对碘番酸的摄取。
DOI：
10.1152/ajpgi.1983.244.6.g630
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Berk,RN
中科院分区：
文献类型：
--
作者：
Barnhart,JL;Witt,BL;Hardison,WG;Berk,RN
采用胶原酶灌流法分离大鼠肝细胞，在原代培养3天后研究碘番酸（IOP）的摄取。用125 I-IOP（1.0-100 μ M）孵育后细胞的125 I活性用作摄取的量度。在每个IOP浓度下，前45 s的吸收呈线性。初始摄取速度与IOP浓度成正比，并且在高达100 μ M时不饱和。计算的摄取速率常数为0.67 nmol。mg蛋白-1。min-1。微M-1。当在4 ℃下进行孵育时，摄取仅略微降低，并且与钠浓度无关。培养基中的白蛋白降低IOP摄取。然而，摄取总是大于单独从未结合IOP浓度预测的摄取。这些数据表明，肝细胞摄取IOP的过程既有被动过程，也有饱和过程。可饱和摄取组分取决于白蛋白-IOP-肝细胞相互作用。白蛋白对摄取的影响可能通过促进IOP摄取的白蛋白的未定义的特定细胞表面现象或通过增强IOP在未搅拌层中的扩散性而发生。
Uptake of iopanoic acid (IOP) was studied in 3-day primary cultures of rat hepatocytes isolated by the collagenase perfusion method. 125I activity of cells after incubation with 125I-IOP (1.0–100 microM) was used as a measure of uptake. At each IOP concentration uptake was linear for the first 45 s. The initial uptake velocity was directly proportional to IOP concentration and was nonsaturable up to 100 microM. The calculated uptake rate constant was 0.67 nmol . mg prot-1 . min-1 . microM-1. Uptake was only slightly reduced when the incubation was performed at 4 degrees C and was independent of sodium concentration. Albumin in the medium reduced IOP uptake. Uptake, however, was always greater than that predicted from the unbound IOP concentration alone. The data indicate that the hepatocyte uptake of IOP occurs by both a passive process and a saturable process. The saturable uptake component depends on an albumin-IOP-hepatocyte interaction. The influence of albumin on uptake occurs possibly by an undefined specific cell surface phenomenon of albumin that promotes uptake of IOP or by enhancement of the diffusibility of IOP across the unstirred layer.