Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon
Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon
复制标题
1-甲基环丙烯和乙烯利处理的采后白花菜 D.C 叶的差异蛋白质谱
DOI：
10.1016/j.foodchem.2014.11.081
复制
发表时间：
2015-06-01
期刊：
影响因子：
8.8
通讯作者：
Yu, Zhifang
中科院分区：
文献类型：
--
作者：
Jiang, Li;Kang, Ruoyi;Yu, Zhifang
从用 1-甲基环丙烯和乙烯利处理过的 G. bicolor 中提取蛋白质，然后在室温下保存 1、3 和 7 天。 2-DE 检测到 300 多个蛋白点，并使用 MALDI-TOF/TOF 切除并分析了 38 个差异丰度点（P < 0.05）。三十三种蛋白质最终被自信地鉴定出来。根据蛋白质直系同源群簇，鉴定出的蛋白质被分为负责新陈代谢（75.8%）、信息存储和处理（9.1%）以及细胞过程和信号传导（12.1%）的蛋白质。与乙烯利和对照处理相比，1-甲基环丙烯特别增加了超氧化物歧化酶、过氧化物酶、碳酸酐酶、核苷二磷酸激酶、甘油醛3-磷酸脱氢酶、RuBisCO和核酮糖二磷酸羧化酶/加氧酶激活酶的丰度。 1-甲基环丙烯通过增强应激反应和防御来保护叶片叶绿体和细胞，并通过抑制物质和能量代谢来延缓衰老。因此，1-甲基环丙烯可以使双色蓼叶具有更好的自卫能力并延缓衰老。 (C) 2015 年，爱思唯尔有限公司出版。
Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7 days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P < 0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. (C) 2015 Published by Elsevier Ltd.