THE EFFECTS OF INHALATION OF GRAIN DUST EXTRACT AND ENDOTOXIN ON UPPER AND LOWER AIRWAYS
THE EFFECTS OF INHALATION OF GRAIN DUST EXTRACT AND ENDOTOXIN ON UPPER AND LOWER AIRWAYS
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DOI：
10.1378/chest.104.3.825
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发表时间：
1993-09-01
期刊：
影响因子：
9.6
通讯作者：
SCHWARTZ, DA
中科院分区：
文献类型：
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作者：
CLAPP, WD;THORNE, PS;SCHWARTZ, DA
为了表征谷物粉尘对肺功能、粘膜炎症和全身反应的短期影响，4名女性和3名男性吸入雾化的玉米和大豆粉尘提取物、用Hanks平衡盐溶液（HBSS）稀释的内毒素和HBSS。受试者是通过报纸广告招募的志愿者，要求是健康、无哮喘、非过敏性从不吸烟者。平均年龄为26.9岁（范围：19 - 36岁）。采用随机、双盲、交叉设计，每例受试者分别接受4种物质的挑战，挑战间隔至少10天。进行连续肺量测定、外周血白细胞和分类细胞计数以及攻毒后24小时鼻灌洗。通过将3g玉米粉或大豆粉与30 ml HBSS混合，随后振荡60分钟，离心，然后过滤灭菌来制备提取物。通过将冻干的大肠杆菌内毒素（血清型0111：B4）与HBSS混合以达到7 mg/L的最终浓度来制备内毒素溶液，该浓度与两种谷物粉尘溶液中的内毒素浓度相同。将所有溶液和未混合的HBSS的pH调节至5.8，这是大豆粉提取物的天然pH。受试者用0.08 ml/kg的每种物质进行挑战，导致内毒素剂量范围为30至60 μ g，类似于工人在一个轮班过程中可能吸入的剂量。吸入激发后，HBSS的最低平均基线FEV百分比（+/- SD）为99.2 +/- 2.1，内毒素显著更低（90.1 +/- 8.5，p = 0.03）、玉米粉提取物（93.1 +/- 4.3，p = 0.02）和大豆粉提取物（96.2 +/- 3.7，p = 0.03）。此外，暴露于所有三种含内毒素的溶液后，外周血白细胞增多（p
To characterize the short-term effects of grain dusts on pulmonary function, mucosal inflammation, and systemic responses, four women and three men inhaled nebulized corn and soybean dust extracts, endotoxin diluted with Hanks' balanced salt solution (HBSS), and HBSS. Subjects were volunteers recruited via newspaper advertisement and were required to be healthy, nonasthmatic, nonatopic never-smokers. The mean age was 26.9 years (range, 19 to 36 years). Using a randomized, double-blind, crossover design, each subject was challenged with each of the 4 substances with at least 10 days between challenges. Serial spirometry, peripheral blood leukocyte and differential cell counts, and 24-h postchallenge nasal lavages were performed. Extracts were produced by mixing 3 g of the corn or soybean dust with 30 ml HBSS followed by shaking for 60 min, centrifugation, then filter sterilization. The endotoxin solution was produced by mixing lyophilized Escherichia coli endotoxin (serotype 0111:B4) with HBSS to attain a final concentation of 7 mg/L, which was the same as the concentration of endotoxin in both grain dust solutions. The pH of all solutions and unmixed HBSS was adjusted to 5.8, which was the native pH of the soybean dust extract. Subjects were challenged with 0.08 ml/kg of each substance, resulting in a range of endotoxin doses of 30 to 60 mug, similar to that which a worker might inhale over the course of one workshift. The lowest mean percentage baseline FEV, (+/- SD) after inhalation challenge was 99.2 +/- 2.1 for HBSS, and it was significantly lower for endotoxin (90.1 +/- 8.5, p = 0.03), corn dust extract (93.1 +/- 4.3, p = 0.02), and soybean dust extract (96.2 +/- 3.7, p = 0.03). In addition, a peripheral blood leukocytosis developed after exposure to all three endotoxin-containing solutions (p