Progesterone receptors in the chick oviduct. Determination of the total concentration of binding sites in the cytosol and nuclear fraction and effect of progesterone on their distribution.
Progesterone receptors in the chick oviduct. Determination of the total concentration of binding sites in the cytosol and nuclear fraction and effect of progesterone on their distribution.
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雏鸡输卵管中的黄体酮受体。
DOI：
10.1111/j.1432-1033.1977.tb11265.x
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
E. Baulieu
中科院分区：
文献类型：
--
作者：
J. Mester;E. Baulieu
1.应用交换技术测定了鸡输卵管胞浆和细胞核中孕酮受体结合位点的总量。2.胞液中孕激素受体的水平受雌激素和孕激素的共同控制。通过苯甲酸雌二醇的初级刺激，在magnum胞质溶胶中受体浓度从约10 000个位点/细胞增加至约40 000个位点/细胞;随后停止雌激素治疗导致6周后降低至约14 000个位点/细胞。孕激素给药（3毫克/公斤）后，雌激素刺激，撤回鸡，受体浓度下降到约40%的初始水平的第一个4小时内，之后的受体水平再次上升，并通过40小时略超过初始的。3.受体的核水平在孕酮注射后1小时达到最大值;然而，细胞核（约900个/细胞核）的结合位点的增加并不能解释它们从胞质溶胶中的损失。孕酮在1-10 mg/kg剂量范围内对核受体最大值无影响。4.核孕酮受体的提取0.5 M氯化钠的强烈影响的预孵育的细胞核在30 ° C，不管这种预孵育，约12%的核受体抵制提取与2 M氯化钠/5 M尿素。
1. Exchange techniques were developed for measurement of total progesterone receptor binding sites concentration in the cytosol and nuclei of chicken oviduct. 2. The level of progesterone receptor in the cytosol was under both oestrogen and progesterone control. Primary stimulation by oestradiol benzoate increased the receptor concentration from approximately 10 000 sites/cell to approximately 40 000 sites/cell in the magnum cytosol; subsequent withdrawal from oestrogen treatment led to a decrease to approximately 14 000 sites/cell after 6 weeks. After progesterone administration (3 mg/kg) to oestrogen-stimulated, withdrawn chicken, the receptor concentration decreased to approximately 40% of the initial level within the first 4 h; afterwards the receptor level rose again and by 40 h exceeded slightly the initial one. 3. The nuclear levels of the receptor reached a maximum at 1 h after the progesterone injection; however, the gain of the binding sites by the nuclei (approximately 900/nucleus) did not account for their loss from the cytosol. The maximum nuclear receptor level was not influenced by the dose of progesterone within range of 1-10 mg/kg. 4. The extractibility of the nuclear progesterone receptor by 0.5 M NaCl was strongly influenced by preincubation of the nuclei at 30 degrees C; irrespective of this preincubation, approximately 12% of the nuclear receptor resisted extraction with 2 M NaCl/5 M urea.