Comparison of the levels of inositol metabolites in transformed haemopoietic cells and their normal counterparts.
Comparison of the levels of inositol metabolites in transformed haemopoietic cells and their normal counterparts.
复制标题
转化造血细胞和正常造血细胞中肌醇代谢物水平的比较。
作者：
C. Bunce;P. French;P. Allen;J. Mountford;B. Moor;M. Greaves;R. Michell;G. Brown
我们比较了三对正常细胞和转化细胞中肌醇代谢物的水平，这些细胞在细胞谱系、分化和增殖状态方面都相匹配：（i）正常人骨髓母细胞和人早幼粒细胞白血病细胞系HL60； (ii) 人脐带 T 辅助细胞和 C8166 细胞，一种 HTLV-1 转化的 T 辅助细胞系； (iii) 鼠原 B 细胞 (BAF3) 和通过 bcr-abl 癌基因转染转化的 BAF3 细胞的白细胞介素 3 依赖性长期培养物。每个细胞群中都存在复杂的肌醇代谢物模式。尽管配对组中各个细胞群之间的某些肌醇代谢物水平存在许多差异，但我们没有观察到增殖的正常细胞和转化细胞之间的肌醇代谢物水平有任何一致的差异。特别是，我们的数据不支持其他工作人员报告的甘油磷酸肌醇（GroPIns）水平升高与膜和细胞质癌基因转化细胞之间的相关性。所有细胞均含有高浓度的 Ins(1,3,4,5,6)P5（12 至 55 µM）和 InsP6（37 至 105 µM）。 HTLV1 转化的 T 辅助细胞具有特别高水平的总肌醇磷酸（主要是 GroPIns、一种未鉴定的肌醇二磷酸和 InsP6）。结合细胞转化和配对群体的分化状态讨论了观察结果。
We have compared the levels of inositol metabolites in three pairs of normal and transformed cells which have been matched with respect to their cell lineage, differentiation and proliferation status: (i) normal human myeloid blast cells and the human promyelocytic leukaemic cell line, HL60; (ii) human umbilical-cord T-helper cells and C8166 cells, a HTLV-1-transformed T-helper cell line; and (iii) an interleukin 3-dependent long-term culture of murine pro-B-cells (BAF3) and BAF3 cells transformed by transfection with the bcr-abl oncogene. Complex patterns of inositol metabolites were present in each of the cell populations. Although there were a number of differences in the levels of certain inositol metabolites between individual cell populations in the paired groups, we did not observe any consistent difference in the levels of inositol metabolites between the proliferating normal and transformed cells. In particular, our data do not support the reported correlation between elevated glycerophosphoinositol (GroPIns) levels and transformation of cells by membrane and cytoplasmic oncogenes which has been reported by other workers. All the cells contained high concentrations of Ins(1,3,4,5,6)P5 (between 12 and 55 microM) and InsP6 (between 37 and 105 microM). The HTLV1-transformed T-helper cells had particularly high levels of total inositol phosphates (predominantly GroPIns, an unidentified inositol bisphosphate and InsP6). The observations are discussed with reference to cell transformation and to the differentiation status of the paired populations.