Mound-cell movement and morphogenesis in Dictyostelium.
Mound-cell movement and morphogenesis in Dictyostelium.
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盘基网柄菌中的丘细胞运动和形态发生。
DOI：
10.1006/dbio.1999.9208
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
McNally,JG
中科院分区：
文献类型：
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作者：
Kellerman,KA;McNally,JG
为了研究三维（3-D）细胞团内细胞运动的机制，我们对Dictyosteliummund中的单个细胞运动进行了系统的3-D分析，Dictyosteliummund是子实体发育过程中形成的第一个3-D结构。我们使用延时去卷积显微镜检查两个菌株，其运动代表我们在土丘中观察到的运动行为谱上的端点。在AX-2土丘中，细胞运动缓慢，轨迹是随机和径向的组合，与KAX-3相比，其中运动快五倍，大多数轨迹是旋转的。虽然径向或旋转运动与每个菌株中存在的光密度波模式相关，但我们也发现了与大多数细胞不同的小但重要的细胞亚群，这表明光密度波充其量不足以解释土丘中的所有运动行为。在研究这些菌株的形态发生时，我们注意到AX-2土丘倾向于直接到达子实体，而KAX-3土丘首先形成迁移蛞蝓。通过改变缓冲条件，我们可以交换这些行为，然后发现丘细胞运动也相应地改变。这表明丘状细胞运动和随后的发育之间存在相关性，但这不是强制性的。嵌合土丘组成的只有10%的KAX-3细胞和90%的AX-2细胞表现出旋转运动，这表明一个可扩散的分子诱导旋转，但许多这些土丘仍然直接达到高潮，表明旋转运动并不总是导致蛞蝓迁移。我们的观察提供了一个详细的分析细胞运动的两种不同模式的土堆和蛞蝓形成inDictyosteelium。
To examine the mechanisms of cell locomotion within a three-dimensional (3-D) cell mass, we have undertaken a systematic 3-D analysis of individual cell movements in theDictyosteliummound, the first 3-D structure to form during development of the fruiting body. We used time-lapse deconvolution microscopy to examine two strains whose motion represents endpoints on the spectrum of motile behaviors that we have observed in mounds. In AX-2 mounds, cell motion is slow and trajectories are a combination of random and radial, compared to KAX-3, in which motion is fivefold faster and most trajectories are rotational. Although radial or rotational motion was correlated with the optical-density wave patterns present in each strain, we also found small but significant subpopulations of cells that moved differently from the majority, demonstrating that optical-density waves are at best insufficient to explain all motile behavior in mounds. In examining morphogenesis in these strains, we noted that AX-2 mounds tended to culminate directly to a fruiting body, whereas KAX-3 mounds first formed a migratory slug. By altering buffering conditions we could interchange these behaviors and then found that mound-cell motions also changed accordingly. This demonstrates a correlation between mound-cell motion and subsequent development, but it is not obligatory. Chimeric mounds composed of only 10% KAX-3 cells and 90% AX-2 cells exhibited rotational motion, suggesting that a diffusible molecule induces rotation, but many of these mounds still culminated directly, demonstrating that rotational motion does not always lead to slug migration. Our observations provide a detailed analysis of cell motion for two distinct modes of mound and slug formation inDictyostelium.