Performance of an enzyme reactor utilizing a magnetic field
Performance of an enzyme reactor utilizing a magnetic field
复制标题
利用磁场的酶反应器的性能
DOI：
10.1002/bit.260220120
复制
发表时间：
1980
影响因子：
3.8
通讯作者：
M. Terashima
中科院分区：
文献类型：
--
作者：
E. Sada;S. Katoh;M. Terashima
在固定化酶的许多应用中，在液膜和固定化珠中都观察到扩散限制。为了降低液相阻力，反应混合物（含有底物和固定化酶）必须剧烈搅拌。然而，剧烈刺激容易导致固定化酶珠的磨损。一些研究人员将酶固定在磁性材料上，并将其用于酶反应器，以促进固定化珠子的磁性分离。在本文中，我们报告了利用旋转磁场的固定化酶反应器性能的初步结果。在该反应器中，避免了珠子与搅拌器叶片碰撞所引起的磨损，并且液相阻力显着降低。
In many of the applications of immobilized enzymes, diffusional restrictions have been observed both in the liquid film and in the immobilized bead. In order to reduce the liquid-phase resistance, the reaction mixture (containing substrate and immobilized enzyme) must be vigorously stirred. Vigorous stimng, however, is liable to cause the attrition of the immobilized-enzyme beads. Some investigators have immobilized enzymes onto magnetic materials and used these for enzyme reactors in order to facilitate the separation of the immobilized beads by magnetic In this paper, we report preliminary results on the performance of an immobilizedenzyme reactor utilizing a revolving magnetic field. In this reactor the attrition of the beads that accompanies collision with a stirrer blade was avoided and the reduction of the liquid-phase resistance was appreciable.