MicroRNA promoter element discovery in Arabidopsis
MicroRNA promoter element discovery in Arabidopsis
复制标题
DOI：
10.1261/rna.130506
复制
发表时间：
2006-09-01
期刊：
影响因子：
4.5
通讯作者：
Hatzigeorgiou, Artemis G.
中科院分区：
文献类型：
--
作者：
Megraw, Molly;Baev, Vesselin;Hatzigeorgiou, Artemis G.
在这项研究中，我们提出了一种方法，确定拟南芥miRNA启动子元件使用已知的转录因子结合基序。我们提供了一个比较分析的代表性，这些元素的miRNA启动子，蛋白质编码基因启动子，和随机基因组序列。我们报告了5个转录因子（TF）结合基序，这些基序在miRNA启动子区相对于蛋白质编码基因的启动子区表现出过度表达的证据。本研究基于对拟南芥中63个实验验证的miRNA初级转录物的转录起始位点（TSS）上游800个核苷酸区域的分析。虽然TATA盒结合基序也由Xie及其同事先前报道，但转录因子AtMYC2，ARF，SORLREP 3和LFY首次被鉴定为miRNA启动子中过度表达的结合基序。
In this study we present a method of identifying Arabidopsis miRNA promoter elements using known transcription factor binding motifs. We provide a comparative analysis of the representation of these elements in miRNA promoters, protein-coding gene promoters, and random genomic sequences. We report five transcription factor (TF) binding motifs that show evidence of overrepresentation in miRNA promoter regions relative to the promoter regions of protein-coding genes. This investigation is based on the analysis of 800-nucleotide regions upstream of 63 experimentally verified Transcription Start Sites (TSS) for miRNA primary transcripts in Arabidopsis. While the TATA-box binding motif was also previously reported by Xie and colleagues, the transcription factors AtMYC2, ARF, SORLREP3, and LFY are identified for the first time as overrepresented binding motifs in miRNA promoters.