Dps protein is related to resistance of Mycobacterium abscessus subsp. massiliense against stressful conditions
Dps protein is related to resistance of Mycobacterium abscessus subsp. massiliense against stressful conditions
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DOI：
10.1007/s00253-020-10586-z
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发表时间：
2020-04-06
影响因子：
5
通讯作者：
Kipnis, Andre
中科院分区：
文献类型：
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作者：
de Alcantara, Nayra Rodrigues;de Oliveira, Fabio Muniz;Kipnis, Andre
脓肿分枝杆菌马氏杆菌（myma）属于脓肿分枝杆菌复合体，是一种快速生长的非结核分枝杆菌。它引起的慢性肺部、皮肤和软组织感染可能难以治疗，因为它对常用的抗微生物药物具有内在的耐药性，使其成为一个严重的世界公共卫生问题。铁是微生物生长所必需的营养物质；然而，过量的时候是有毒的。因此，细菌需要铁稳态机制才能在不同的环境中取得成功。来自饥饿细胞（Dps）的DNA结合蛋白是一种微量铁蛋白，具有作为额外的铁储存蛋白的特性，但也可以与DNA结合，保护DNA免受羟基自由基的侵害。对Mycma基因组的注释显示，与来自M. smegmatis mc(2) 155的MSMEG_3242基因相比，mycma_03135基因序列同源性为79%，该基因编码已知的Dps。重组脓肿分枝杆菌Dps （rMaDps）在大肠杆菌中产生，以可溶性形式纯化，并在溶液中形成高质量的低聚物，具有铁氧化酶活性，DNA结合和抗损伤保护作用。过氧化氢（H2O2）诱导mycma_03135基因在Mycma生长过程中表达。此外，大肠杆菌表达的rMaDps对H2O2具有更强的抗性。因此，这项研究是第一个鉴定和表征脓肿分枝杆菌的Dps。
Mycobacterium abscessus subsp. massiliense (Mycma) belongs to the Mycobacterium abscessus complex and is a rapidly growing non-tuberculous mycobacterium. The chronic pulmonary, skin, and soft tissue infections that it causes may be difficult to treat due to its intrinsic resistance to the commonly used antimicrobial drugs, making it a serious world public health problem. Iron is an essential nutrient for the growth of microorganisms; nonetheless, it can be toxic when in excess. Thus, bacteria require an iron homeostasis mechanism to succeed in different environments. DNA-binding proteins from starved cells (Dps) are miniferritins with the property to act as additional iron storage proteins but also can bind to DNA, protecting it against hydroxyl radical. Annotation of the Mycma genome revealed the gene mycma_03135 with 79% sequential identity when compared to MSMEG_3242 gene from M. smegmatis mc(2) 155, which codifies for a known Dps. Recombinant Dps from M. abscessus (rMaDps) was produced in Escherichia coli, purified in soluble form and shown to form high mass oligomers in solution with ferroxidase activity, DNA binding, and protection against damage. The expression of the mycma_03135 gene was induced during Mycma growth in the presence of hydrogen peroxide (H2O2). Additionally, the expression of rMaDps by E. coli conferred greater resistance to H2O2. Thus, this study is the first to identify and characterize a Dps from M. abscessus.