Diversity and Heterogeneity of Epibiotic Bacterial Communities on the Marine Nematode Eubostrichus dianae
Diversity and Heterogeneity of Epibiotic Bacterial Communities on the Marine Nematode Eubostrichus dianae
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DOI：
10.1128/aem.65.9.4271-4275.1999
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发表时间：
1999-09
影响因子：
4.4
通讯作者：
M. Polz;Christopher W. Harbison;C. Cavanaugh
中科院分区：
文献类型：
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作者：
M. Polz;Christopher W. Harbison;C. Cavanaugh
摘要利用16SrDNA酶切和测序技术分析了海洋线虫体表微生物群落的多样性。在两个克隆文库构建通过使用单独的线虫，54和85限制性模式进行了鉴定，只有13这些模式是共同的两个库。序列分析表明，共同的模式属于4组相关的序列的噬细胞，硫酸盐还原菌，成员的γ亚类的类变形菌，和柄杆菌。至少有两个群体似乎是永久成员的社会，因为他们也被发现在16S rDNA文库构建3年前使用100个汇集的线虫标本。一个令人惊讶的结果是，非常占优势的丝状细菌显然没有在克隆文库中表示，因为定量探测显示，没有一个常见的操作分类单元组显示出预期的压倒性优势。然而，我们的分析揭示了在代表可能非常相似的微环境的样品中出乎意料的高水平的细菌多样性和异质性。
ABSTRACT The diversity of a microbial community covering the surface of a marine nematode was analyzed by performing a 16S ribosomal DNA (rDNA) restriction cutting and sequencing analysis. In two clone libraries constructed by using individual nematodes, 54 and 85 restriction patterns were identified, and only 13 of these patterns were common to both libraries. Sequence analysis indicated that the common patterns belonged to four groups related to sequences of cytophagas, sulfate-reducing bacteria, members of the gamma subclass of the classProteobacteria, and caulobacters. At least two groups appeared to be permanent members of the community as they were also detected in a 16S rDNA library constructed 3 years previously by using 100 pooled nematode specimens. A surprising outcome was that very dominant filamentous bacteria were apparently not represented in the clone libraries, as quantitative probing showed that none of the common operational taxonomic unit groups displayed the expected overwhelming dominance. Nevertheless, our analysis revealed both an unexpectedly high level of bacterial diversity and heterogeneity in samples representing presumably very similar microenvironments.