Influence of bisphosphonates on orthodontic tooth movement in mice
Influence of bisphosphonates on orthodontic tooth movement in mice
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DOI：
10.1093/ejo/cjp068
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发表时间：
2009-12-01
影响因子：
2.6
通讯作者：
Yoshida, Noriaki
中科院分区：
文献类型：
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作者：
Fujimura, Yuji;Kitaura, Hideki;Yoshida, Noriaki
在8周龄雄性C57BL/6小鼠上前牙槽骨和第一磨牙之间插入一个力为10 g的镍钛封闭线圈弹簧。每天将双膦酸盐（2 μ g/20 μ l）注射到上磨牙附近的局部部位。12天后，测量牙齿移动的距离。在组织学切片中，将抗酒石酸酸性磷酸酶（TRAP）阳性细胞计数为破骨细胞。用扫描电镜观察根吸收情况。数据用学生t检验进行分析。正畸矫治器增加了压力侧破骨细胞的数量和第一磨牙的近内侧运动。双膦酸盐降低了牙齿的运动量和破骨细胞的数量。此外，它们还减少了压力侧的根吸收。双膦酸盐抑制小鼠正畸牙齿运动，阻止正畸牙齿运动时牙根吸收。这些结果表明，双膦酸盐可能对人类正畸牙齿运动过程中的牙根吸收有抑制作用，并且可能会中断正在接受治疗的正畸患者的牙齿运动，从而改变治疗结果。
A nickel-titanium (Ni-Ti) closed coil spring delivering a force of 10 g was inserted between the upper anterior alveolar bone and the first molar in 8-week-old male C57BL/6 mice. Bisphosphonate (2 mu g/20 mu l) was injected daily into a local site adjacent to the upper molar. After 12 days, the distance the tooth had moved was measured. The number of tartrate-resistant acid phosphatase (TRAP)-positive cells was counted as osteoclasts in histological sections. Root resorption was assessed by scanning electron microscopy. The data were analysed with a Student's t-test.The orthodontic appliance increased the number of osteoclasts on the pressure side and mesial movement of the first molar. Bisphosphonates reduced the amount of tooth movement and the number of osteoclasts. In addition, they also reduced root resorption on the pressure side.Bisphosphonates inhibit orthodontic tooth movement and prevent root resorption during orthodontic tooth movement in mice. These results suggest that bisphosphonates might have an inhibiting effect on root resorption during orthodontic tooth movement in humans and that they may interrupt tooth movement in orthodontic patients undergoing treatment, thus altering the outcome of treatment.