EVALUATION OF MONOCLONAL-ANTIBODIES HAVING SPECIFICITY FOR HUMAN-IGG SUBCLASSES - RESULTS OF THE 2ND IUIS WHO COLLABORATIVE STUDY
EVALUATION OF MONOCLONAL-ANTIBODIES HAVING SPECIFICITY FOR HUMAN-IGG SUBCLASSES - RESULTS OF THE 2ND IUIS WHO COLLABORATIVE STUDY
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DOI：
10.1016/0165-2478(92)90141-a
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发表时间：
1992-02-01
影响因子：
4.4
通讯作者：
COOLEN, J
中科院分区：
文献类型：
--
作者：
JEFFERIS, R;REIMER, CB;COOLEN, J
在单克隆抗IgG亚类抗体的第一次IUIS/WHO合作研究之后，建立了一组WHO特异性参考试剂（SRR）[Jefferis，R.，等人（1985）免疫学快报，10，223]。当时，人们希望能够获得更多的试剂，特别是针对IgG 2和其他同种异型特异性的试剂，这些试剂可以应用于广泛的测定方案中。第二项研究报告了19种抗亚类和7种抗同种异型单克隆抗体的评价。抗IgG 1抗体HP 6187的性能与SRR相当。其他的，不是小鼠IgG 1同种型，可能是有用的特定应用。抗IgG 2抗体HP 6200可能是WHO SRR的有价值的补充;它对Fab区中的表位具有特异性，但不具有HP 6014的轻链偏倚。当在与原始实验室所采用的方案相似的方案中使用时，推定同种异型特异性的抗体表现出声明的特异性。这似乎是固有的性质的表位（allotopes）认识到，这将需要几年的试剂适用于广泛的技术将成为可用。
Following the 1st IUIS/WHO Collaborative Study of monoclonal anti-IgG subclass antibodies, a panel of WHO Specificity Reference Reagents (SRR) was established [Jefferis, R., et al. (1985) Immunol. Lett., 10, 223]. At the time, the hope was expressed that further reagents particularly for IgG2, and other allotypic specificities would become available which could be applied in a wide range of assay protocols. The 2nd study reports the evaluation of nineteen anti-subclass and seven anti-allotype monoclonal antibodies. The anti-IgG1 antibody HP6187 was equivalent in performance to the SRR. Others, that were not of the mouse IgG1 isotype, may be useful for particular applications. The anti-IgG2 antibody HP6200 could be a valuable addition to the WHO SRR; it is specific for an epitope in the Fab region but does not have the light chain bias of HP6014. Antibodies of putative allotype specificity exhibited the claimed specificity when used within protocols similar to those employed by the originating laboratory. It appears to be inherent in the nature of the epitopes (allotopes) recognized that it will take several years before reagents applicable to a wide range of techniques will become available.