Multiple virus infections in the honey bee and genome divergence of honey bee viruses
Multiple virus infections in the honey bee and genome divergence of honey bee viruses
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DOI：
10.1016/j.jip.2004.07.005
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发表时间：
2004-10-01
影响因子：
3.4
通讯作者：
Feldlaufer, M
中科院分区：
文献类型：
--
作者：
Chen, YP;Zhao, Y;Feldlaufer, M
我们利用单重逆转录聚合酶链反应（uniplex RT - PCR）对蜜蜂蜂群进行了几种蜜蜂病毒的筛查，包括黑蜂王台病毒（BQCV）、残翅病毒（DWV）、克什米尔蜜蜂病毒（KBV）和囊状幼虫病毒（SBV），并描述了在这些蜂群的蜜蜂中检测到混合病毒感染的情况。我们首次报道单只蜜蜂可同时携带四种病毒。我们还开发了一种多重逆转录聚合酶链反应（multiplex RT - PCR）检测方法，用于同时检测多种蜜蜂病毒。多重逆转录聚合酶链反应检测方法的可行性和特异性表明，该方法是一种同时检测蜂群中混合病毒感染的有效工具，对于在田间和实验室诊断和监测蜜蜂病毒性疾病将很有用。对病毒编码的假定解旋酶和依赖RNA的RNA聚合酶（RdRp）进行的系统发育分析显示，DWV和SBV属于同一分支，而KBV和BQCV与感染植物、昆虫和脊椎动物的其他小RNA病毒样病毒属于不同的谱系。对这些病毒的田间调查结果表明，蜜蜂中BQCV、DWV、KBV和SBV的混合感染可能是由于病毒广泛的地理分布所致。由爱思唯尔公司出版。
Using uniplex RT-PCR we screened honey bee colonies for the presence of several bee viruses, including black queen cell virus (BQCV), deformed wing virus (DWV), Kashmir bee virus (KBV), and sacbrood virus (SBV), and described the detection of mixed virus infections in bees from these colonies. We report for the first time that individual bees can harbor four viruses simultaneously. We also developed a multiplex RT-PCR assay for the simultaneous detection of multiple bee viruses. The feasibility and specificity of the multiplex RT-PCR assay suggests that this assay is an effective tool for simultaneous examination of mixed virus infections in bee colonies and would be useful for the diagnosis and surveillance of honey bee viral diseases in the field and laboratory. Phylogenetic analysis of putative helicase and RNA-dependent RNA polymerase (RdRp) encoded by viruses reveal that DWV and SBV fall into a same clade, whereas KBV and BQCV belong to a distinct lineage with other picoma-like viruses that infect plants, insects and vertebrates. Results from field surveys of these viruses indicate that mixed infections of BQCV, DWV, KBV, and SBV in the honey bee probably arise due to broad geographic distribution of viruses. Published by Elsevier Inc.