Selective determination of tobacco-specific nitrosamines in mainstream cigarette smoke by GC coupled to positive chemical ionization triple quadrupole MS.
Selective determination of tobacco-specific nitrosamines in mainstream cigarette smoke by GC coupled to positive chemical ionization triple quadrupole MS.
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DOI：
10.1002/jssc.201300030
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发表时间：
2013-08
影响因子：
3.1
通讯作者：
Da Wu;Yifeng Lu;huaqing lin;Wanhong Zhou;Wenbo Gu
中科院分区：
文献类型：
--
作者：
Da Wu;Yifeng Lu;huaqing lin;Wanhong Zhou;Wenbo Gu
建立了一种快速测定烟草特有亚硝胺N-亚硝基去甲烟碱、N-亚硝基新烟草碱、N-亚硝基假木贼碱和4-亚硝基烟碱的方法。1-甲基亚硝胺（3-吡啶基）-1-丁酮，通过气相色谱-正化学电离三重四极杆质谱联用开发。在剑桥滤垫上收集主流香烟烟雾后，用0.1M HCL水溶液提取颗粒物质，通过阳离子交换固相萃取净化，最后进样到GC-MS/MS中，使用同位素标记的类似物作为内标。在0.5-200.0 ng mL（-1）的浓度范围内，所有烟草特有的亚硝胺具有良好的线性关系，相关系数在0.9996-0.9999之间。每种烟草特有亚硝胺的检测限为0.023-0.028 ng cig（-1），定量下限为0.077-0.093 ng cig（-1）。各烟草特有亚硝胺的回收率在90.0- 109.0%之间。日内和日间精密度的相对标准偏差分别为3.1-5.8和3.9-6.6。该方法适用于参考和国产卷烟。结果表明，该方法与传统方法一致，可作为烟草特有亚硝胺的常规分析方法。
A rapid method for the selective determination of four kinds of tobacco-specific nitrosamines, N-nitrosonornicotine, N-nitrosoanatabine, N-nitrosoanabasine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, in mainstream cigarette smoke was developed by GC coupled to positive chemical ionization triple-quadrupole MS. After mainstream cigarette smoke was collected on a cambridge filter pad, the particulate matter was extracted with 0.1 M HCL aqueous solution, cleaned by positive cation-exchange solid extraction, and finally injected into GC-MS/MS using isotopically labeled analogues as internal standards. Excellent linearity was obtained over the concentration range of 0.5-200.0 ng mL(-1) for all tobacco-specific nitrosamines with values for correlation coefficient between 0.9996-0.9999. Limits of detection of each tobacco specific nitrosamine varied from 0.023-0.028 ng cig(-1), and lower limits of quantification varied from 0.077-0.093 ng cig(-1). The recovery of each tobacco specific nitrosamine was from 90.0-109.0%. The relative standard deviations of the intra-day and inter-day precisions were 3.1-5.8 and 3.9-6.6, respectively. This method was applied to reference and domestic cigarettes. The result showed that the method was consistent with traditional methods and can be used as an effective approach for the routine analysis of tobacco-specific nitrosamines.