PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations
PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations
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DOI：
10.1007/978-1-4939-9432-8_11
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Kato, Takamitsu A.
中科院分区：
文献类型：
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作者：
Cartwright, Ian M.;Haskins, Jeremy S.;Kato, Takamitsu A.
双着丝粒和着丝粒环染色体用于辐射损伤分析和辐射照射后的生物剂量测定。然而，基于Giemsa染色的细胞遗传学分析是劳动密集型和耗时的。此外，基于Giemsa的染色体分析的缺点是当研究人员没有经过充分的分析训练时，潜在的再现性差。这些问题来自于染色体畸变的形态异常分析。基因座特异性FISH探针被用来克服这个问题。着丝粒探针可以可视化着丝粒的位置，并帮助识别双着丝粒染色体和着丝粒环。端粒探针有助于识别末端缺失和端粒融合。探针最初是用DNA探针设计的，但肽核酸（PNA）探针取代了DNA探针。本章介绍了PNA端粒和着丝粒FISH染色及染色体畸变的准确分析。
Dicentric and centric ring chromosomes are used for radiation-induced damage analysis and biodosimetry after radiation exposure. However, Giemsa stain-based cytogenetic analysis is labor-intense and time-consuming. Moreover, the disadvantage of Giemsa based chromosome analysis is a potential poor reproducibility when researchers are not fully trained for analysis. These problems come from analysis of morphological abnormality of chromosomal aberrations. Locus-specific FISH probes were used to overcome this problem. Centromere probes can visualize centromere locations and help identify dicentric chromosomes and centric rings. Telomere probes help to identify terminal deletion and telomere fusions. Probes were originally designed with a DNA probe but Peptide nucleic acid (PNA) probes took the place of DNA probes. This chapter introduces PNA telomere and centromere FISH staining and accurate analysis of chromosomal aberrations.