Applications of thin layer chromatography, high performance liquid chromatography and mass spectrometry in the fermentation and isolation of the antibiotic nybomycin
Applications of thin layer chromatography, high performance liquid chromatography and mass spectrometry in the fermentation and isolation of the antibiotic nybomycin
复制标题
薄层色谱、高效液相色谱和质谱技术在抗生素新霉素发酵分离中的应用
DOI：
--
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
R. C. Pandey
中科院分区：
文献类型：
--
作者：
J. Pope;R. A. Nelson;C. Schaffner;R. T. Rosen;R. C. Pandey
本文建立了抗生素尼波霉素及其衍生物脱氧尼波霉素和尼波霉素醋酸酯在发酵和分离过程中的薄层色谱（TLC）、高效液相色谱（HPLC）和质谱（MS）分析方法。使用基于定量HPLC的测定法，测定1000升发酵中尼博霉素生产的时间过程（尼博霉素滴度）。解吸化学电离质谱法（DCI/MS）的标准尼波霉素样品，发酵液样品和纯化的馏分表明，共同生产的脱氧尼波霉素，这是以前没有报道从这种生物体。采用TLC和HPLC法对发酵液粗提物中脱氧尼波霉素的存在进行了确证。
SummaryThin layer chromatography (TLC), high performance liquid chromatography (HPLC) and mass spectrometry (MS) methods have been developed for the analysis of the antibiotic nybomycin, its derivatives deoxynybomycin and nybomycin acetate, during the fermentation and isolation of nybomycin. Using a quantitative HPLC based assay, the time course of nybomycin production (nybomycin titers) in 1000 liter fermentations was determined. Desorption chemical ionization mass spectrometry (DCI/MS) of standard nybomycin samples, fermentation broth samples and purified fractions suggested the co-production of deoxynybomycin which was not reported previously from this organism. TLC and HPLC were used to confirm the presence of deoxynybomycin in the crude extracts of fermentation broths.