Complete nucleotide sequence of insertion element IS4351 from Bacteroides fragilis.
Complete nucleotide sequence of insertion element IS4351 from Bacteroides fragilis.
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来自脆弱拟杆菌的插入元件 IS4351 的完整核苷酸序列。
DOI：
10.1128/jb.169.8.3573-3580.1987
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发表时间：
1987
影响因子：
3.2
通讯作者：
Macrina,FL
中科院分区：
文献类型：
--
作者：
Rasmussen,JL;Odelson,DA;Macrina,FL
对脆弱拟杆菌R质粒pBF 4中大环内酯-林可酰胺-链阳性菌素B耐药决定簇ermF侧翼的一个直接重复序列进行核苷酸序列测定和遗传分析，表明该区域是一个插入序列（IS）元件。这个1,155个碱基对的元件包含部分匹配的（25个碱基对中的20个）末端反向重复序列、重叠的反平行开放阅读框架和9个启动子样序列，其中3个是向外的。该序列的分析表明，没有显着的核苷酸同源性，其他13个已知的IS元件。由于Southern印迹杂交分析检测到染色体DNA中的同源序列，其G+C含量（42 mol%）与B相似。fragilis，数据表明该元素是拟杆菌属起源的。该元件促进的转座在recA E中得到证实。杆菌通过选择质粒pDH 5110上无启动子的氯霉素抗性基因的激活来回收突变体，并通过限制性内切酶图谱和Southern印迹杂交来表征。我们建议将该IS元件命名为IS 4351。
The nucleotide sequence and genetic analyses of one of the directly repeated sequences flanking the macrolide-lincosamide-streptogramin B drug resistance determinant, ermF, from the Bacteroides fragilis R plasmid, pBF4, suggested that this region is an insertion sequence (IS) element. This 1,155-base-pair element contained partially matched (20 of 25 base pairs) terminal-inverted repeats, overlapping, anti-parallel open reading frames, and nine promoterlike sequences, including three that were oriented outward. Analysis of this sequence revealed no significant nucleotide homology to 13 other known IS elements. Inasmuch as Southern blot hybridization analysis detected homologous sequences in chromosomal DNA and its G+C content (42 mol%) was similar to that of B. fragilis, the data suggested that this element is of Bacteroides origin. Transposition promoted by this element was demonstrated in recA E. coli. Recombinants were recovered by selecting for the activation of a promoterless chloramphenicol resistance gene on the plasmid pDH5110 and were characterized by restriction endonuclease mapping and Southern blot hybridization. We propose that this IS element be designated IS4351.