Oxidative stress and skeletal muscle dysfunction are present in healthy smokers.
Oxidative stress and skeletal muscle dysfunction are present in healthy smokers.
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DOI：
10.1590/1414-431x20165512
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发表时间：
2016-10-24
期刊：
影响因子：
--
通讯作者：
Mendonça VA
中科院分区：
文献类型：
--
作者：
Neves CD;Lacerda AC;Lage VK;Lima LP;Tossige-Gomes R;Fonseca SF;Rocha-Vieira E;Teixeira MM;Mendonça VA
慢性暴露于香烟烟雾似乎与促炎细胞因子的增加、氧化应激以及健康吸烟者的肌肉和身体表现的变化有关。然而，这些参数尚未在以前的研究中同时进行评价。本研究的参与者是健康男性，分为两组：吸烟者（n=20）和非吸烟者（n=20）。通过测量细胞因子IL-10、IL-6 e TNF-α以及可溶性受体sTNFR 1和sTNFR 2的血浆水平来评价炎症。通过测定硫代巴比妥酸反应物质（TBARS）血浆水平、血浆总抗氧化能力和红细胞抗氧化酶超氧化物歧化酶（SOD）和过氧化氢酶活性来评估氧化应激。通过测量膝关节屈肌和伸肌的峰值扭矩以及确定膝关节伸肌的总功来评价肌肉性能。通过测定最大摄氧量（VO 2 peak）、最大心率（HRmax）和往返步行试验中的步行距离来评估体能。吸烟者血浆总抗氧化能力、过氧化氢酶活性和总功均降低（P<0.05），sTNFR 1和TBARS水平升高（P<0.05）。IL-6、IL-10、sTNFR 2、SOD、峰值扭矩、VO 2峰值、HRmax和步行距离在组间相似。吸烟者表现出增加的氧化应激和骨骼肌功能障碍，表明在健康吸烟者中分子和肌肉参数的变化同时发生。
Chronic exposure to cigarette smoke seems to be related to an increase of pro-inflammatory cytokines, oxidative stress and changes in muscular and physical performances of healthy smokers. However, these parameters have not yet been evaluated simultaneously in previous studies. The participants of this study were healthy males divided into two groups: smokers (n=20) and non-smokers (n=20). Inflammation was evaluated by measuring plasma levels of the cytokines IL-10, IL-6 e TNF-α, and of the soluble receptors sTNFR1 and sTNFR2. Oxidative stress was evaluated by determination of thiobarbituric acid reactive substances (TBARS) plasma levels, total antioxidant capacity of plasma and erythrocytes activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase. Muscular performance was evaluated by measuring the peak torque of knee flexors and extensors, and by determining the total work of the knee extensors. Physical performance was assessed by measuring the peak oxygen uptake (VO2 peak), the maximum heart rate (HRmax) and the walking distance in the shuttle walking test. Smokers showed an increase in the levels of the sTNFR1 and TBARS and a decrease in the total antioxidant capacity of plasma, in the catalase activity and in the total work (P<0.05). IL-6, IL-10, sTNFR2, SOD, peak torque, VO2 peak, HRmax and walking distance were similar between groups. Smokers presented increased oxidative stress and skeletal muscle dysfunction, demonstrating that the changes in molecular and muscular parameters occur simultaneously in healthy smokers.