A micro-liter viscosity and density sensor for the rheological characterization of DNA solutions in the kilo-hertz range
A micro-liter viscosity and density sensor for the rheological characterization of DNA solutions in the kilo-hertz range
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DOI：
10.1039/c3lc50857a
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发表时间：
2013-01-01
期刊：
影响因子：
6.1
通讯作者：
Dual, Jurg
中科院分区：
文献类型：
--
作者：
Rust, Philipp;Cereghetti, Damiano;Dual, Jurg
当测量来自生物源的流体的性质时，通常需要微升范围内的样品体积，因为更高的体积可能不可用或非常昂贵。基于振动悬臂梁的微型粘度和密度传感器满足了这一要求。在本文中，在同一时间使用这样的传感器测量DNA溶液的粘度和密度的可能性。该传感器需要10 μ l的样品体积。通过在稀释的牛顿体系中滴定含有110 bp长链DNA的溶液，使用悬臂梁传感器可以确定特性粘度为0.047 ml mg（-1）。悬臂也测试了10 kbp长链的溶液中的浓度在半稀释，非牛顿制度。使用这些溶液在12.5 kHz下观察到的共振频率和阻尼的微小变化归因于剪切稀化，这是在外推其他组的结果时预期的。
When measuring the properties of fluids from biological sources, sample volumes in the micro-liter range are often desired as higher volumes may not be available or are very expensive. Miniaturized viscosity and density sensors based on a vibrating cantilever fulfill this requirement. In this paper, the possibility of measuring viscosity and density of DNA solutions at the same time using such a sensor is shown. The sensor requires a sample volume of 10 mu l. By doing a titration of a solution containing 110 bp long strands of DNA in the diluted, Newtonian regime, the intrinsic viscosity can be determined to be 0.047 ml mg(-1) using the cantilever sensor. The cantilever is also tested with solutions of 10 kbp long strands with concentrations in the semi-dilute, non-Newtonian regime. The comparably small change in resonance frequency and damping observed using these solutions at 12.5 kHz is attributed to shear thinning, which is expected when extrapolating results from other groups.